Detection of bacterial DNA by PCR in dogs with stifle pathology

Jitender Bhandal, Kei Hayashi, Sun Young Kim, Martha Klein, Alice Wong, Chrisoula A. Toupadakis, Peter Muir, Clare E Yellowley-genetos

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: To determine presence of bacterial DNA in canine stifles with cranial cruciate ligament rupture (CCLR) and medial patellar luxation (MPL) compared to normal canine stifles (control). Study design: Prospective clinical study. Animals: Dogs (n=44). Methods: Dogs of varying age, breed, sex, and weight residing in California were assessed for stifle pathology (CCLR, MPL, or normal control). Synovial fluid of all stifles was assessed for the presence of bacterial DNA using broad-ranging 16S rRNA primers and PCR. Results: Bacterial DNA was detected in normal control stifles and those with CCLR and MPL. There were no statistical differences in the copy numbers of bacterial DNA in the stifle synovial fluid among groups (P>.05); however, synovial fluid specimens from dogs with stifle pathology (CCLR and MPL combined) tended to have higher copy numbers of bacterial DNA than those from controls (P=06). There was no significant difference in the number of bacterial DNA between the CCLR and MPL groups (P=57). The copy numbers of bacterial DNA had a weak positive significant correlation with the duration of lameness in CCLR group (P<.05). Conclusions: Increased detection of bacterial DNA in the stifle synovial fluid may indicate joint pathology but not be directly linked to a specific joint disease.

Original languageEnglish (US)
Pages (from-to)814-818
Number of pages5
JournalVeterinary Surgery
Volume42
Issue number7
DOIs
StatePublished - Oct 2013

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Stifle
Bacterial DNA
cranial cruciate ligament
Anterior Cruciate Ligament
Dogs
Pathology
Rupture
Polymerase Chain Reaction
synovial fluid
dogs
Synovial Fluid
DNA
Canidae
joint diseases
Joint Diseases
lameness
clinical trials
Joints
experimental design
ribosomal RNA

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Detection of bacterial DNA by PCR in dogs with stifle pathology. / Bhandal, Jitender; Hayashi, Kei; Kim, Sun Young; Klein, Martha; Wong, Alice; Toupadakis, Chrisoula A.; Muir, Peter; Yellowley-genetos, Clare E.

In: Veterinary Surgery, Vol. 42, No. 7, 10.2013, p. 814-818.

Research output: Contribution to journalArticle

Bhandal, J, Hayashi, K, Kim, SY, Klein, M, Wong, A, Toupadakis, CA, Muir, P & Yellowley-genetos, CE 2013, 'Detection of bacterial DNA by PCR in dogs with stifle pathology', Veterinary Surgery, vol. 42, no. 7, pp. 814-818. https://doi.org/10.1111/j.1532-950X.2013.12038.x
Bhandal J, Hayashi K, Kim SY, Klein M, Wong A, Toupadakis CA et al. Detection of bacterial DNA by PCR in dogs with stifle pathology. Veterinary Surgery. 2013 Oct;42(7):814-818. https://doi.org/10.1111/j.1532-950X.2013.12038.x
Bhandal, Jitender ; Hayashi, Kei ; Kim, Sun Young ; Klein, Martha ; Wong, Alice ; Toupadakis, Chrisoula A. ; Muir, Peter ; Yellowley-genetos, Clare E. / Detection of bacterial DNA by PCR in dogs with stifle pathology. In: Veterinary Surgery. 2013 ; Vol. 42, No. 7. pp. 814-818.
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