Abstract
Objective: To determine presence of bacterial DNA in canine stifles with cranial cruciate ligament rupture (CCLR) and medial patellar luxation (MPL) compared to normal canine stifles (control). Study design: Prospective clinical study. Animals: Dogs (n=44). Methods: Dogs of varying age, breed, sex, and weight residing in California were assessed for stifle pathology (CCLR, MPL, or normal control). Synovial fluid of all stifles was assessed for the presence of bacterial DNA using broad-ranging 16S rRNA primers and PCR. Results: Bacterial DNA was detected in normal control stifles and those with CCLR and MPL. There were no statistical differences in the copy numbers of bacterial DNA in the stifle synovial fluid among groups (P>.05); however, synovial fluid specimens from dogs with stifle pathology (CCLR and MPL combined) tended to have higher copy numbers of bacterial DNA than those from controls (P=06). There was no significant difference in the number of bacterial DNA between the CCLR and MPL groups (P=57). The copy numbers of bacterial DNA had a weak positive significant correlation with the duration of lameness in CCLR group (P<.05). Conclusions: Increased detection of bacterial DNA in the stifle synovial fluid may indicate joint pathology but not be directly linked to a specific joint disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 814-818 |
| Number of pages | 5 |
| Journal | Veterinary Surgery |
| Volume | 42 |
| Issue number | 7 |
| DOIs | |
| State | Published - Oct 2013 |
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ASJC Scopus subject areas
- veterinary(all)
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Detection of bacterial DNA by PCR in dogs with stifle pathology. / Bhandal, Jitender; Hayashi, Kei; Kim, Sun Young; Klein, Martha; Wong, Alice; Toupadakis, Chrisoula A.; Muir, Peter; Yellowley-genetos, Clare E.
In: Veterinary Surgery, Vol. 42, No. 7, 10.2013, p. 814-818.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of bacterial DNA by PCR in dogs with stifle pathology
AU - Bhandal, Jitender
AU - Hayashi, Kei
AU - Kim, Sun Young
AU - Klein, Martha
AU - Wong, Alice
AU - Toupadakis, Chrisoula A.
AU - Muir, Peter
AU - Yellowley-genetos, Clare E
PY - 2013/10
Y1 - 2013/10
N2 - Objective: To determine presence of bacterial DNA in canine stifles with cranial cruciate ligament rupture (CCLR) and medial patellar luxation (MPL) compared to normal canine stifles (control). Study design: Prospective clinical study. Animals: Dogs (n=44). Methods: Dogs of varying age, breed, sex, and weight residing in California were assessed for stifle pathology (CCLR, MPL, or normal control). Synovial fluid of all stifles was assessed for the presence of bacterial DNA using broad-ranging 16S rRNA primers and PCR. Results: Bacterial DNA was detected in normal control stifles and those with CCLR and MPL. There were no statistical differences in the copy numbers of bacterial DNA in the stifle synovial fluid among groups (P>.05); however, synovial fluid specimens from dogs with stifle pathology (CCLR and MPL combined) tended to have higher copy numbers of bacterial DNA than those from controls (P=06). There was no significant difference in the number of bacterial DNA between the CCLR and MPL groups (P=57). The copy numbers of bacterial DNA had a weak positive significant correlation with the duration of lameness in CCLR group (P<.05). Conclusions: Increased detection of bacterial DNA in the stifle synovial fluid may indicate joint pathology but not be directly linked to a specific joint disease.
AB - Objective: To determine presence of bacterial DNA in canine stifles with cranial cruciate ligament rupture (CCLR) and medial patellar luxation (MPL) compared to normal canine stifles (control). Study design: Prospective clinical study. Animals: Dogs (n=44). Methods: Dogs of varying age, breed, sex, and weight residing in California were assessed for stifle pathology (CCLR, MPL, or normal control). Synovial fluid of all stifles was assessed for the presence of bacterial DNA using broad-ranging 16S rRNA primers and PCR. Results: Bacterial DNA was detected in normal control stifles and those with CCLR and MPL. There were no statistical differences in the copy numbers of bacterial DNA in the stifle synovial fluid among groups (P>.05); however, synovial fluid specimens from dogs with stifle pathology (CCLR and MPL combined) tended to have higher copy numbers of bacterial DNA than those from controls (P=06). There was no significant difference in the number of bacterial DNA between the CCLR and MPL groups (P=57). The copy numbers of bacterial DNA had a weak positive significant correlation with the duration of lameness in CCLR group (P<.05). Conclusions: Increased detection of bacterial DNA in the stifle synovial fluid may indicate joint pathology but not be directly linked to a specific joint disease.
UR - http://www.scopus.com/inward/record.url?scp=84884982019&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884982019&partnerID=8YFLogxK
U2 - 10.1111/j.1532-950X.2013.12038.x
DO - 10.1111/j.1532-950X.2013.12038.x
M3 - Article
C2 - 24033641
AN - SCOPUS:84884982019
VL - 42
SP - 814
EP - 818
JO - Veterinary Surgery
JF - Veterinary Surgery
SN - 0161-3499
IS - 7
ER -