Detection of autoantibodies to recombinant mitochondrial proteins in patients with primary biliary cirrhosis

Judith A Van de Water, A. Cooper, C. D. Surh, R. Coppel, D. Danner, A. Ansari, R. Dickson, M. Eric Gershwin

Research output: Contribution to journalArticle

173 Scopus citations

Abstract

Primary biliary cirrhosis is characterized by the presence of autoantibodies to mitochondria with specific reactivity to proteins of 74 and 52 kilodaltons (kd). The 74-kd mitochondrial protein is the E2 component - dihydrolipoamide acetyltransferase - of the pyruvate dehydrogenase complex, and the 52-kd protein is the equivalent E2 component - dihydrolipoamide acyltransferase - of the branched chain α-keto acid dehydrogenase complex. Current methods for the detection of antibodies to these proteins lack specificity or sensitivity, or they are time-consuming and not readily available. We therefore developed an enzyme-linked immunoassay to quantify specific antimitochondrial antibodies in patients with primary biliary cirrhosis. Recombinant polypeptides coding for both the 74-kd and the 52-kd mitochondrial autoantigens were used to analyze 217 coded serum samples, including samples from 93 patients with primary biliary cirrhosis and 124 controls, for reactivity by our immunoassay, immunoblotting, and immunofluorescence testing. Serum samples from 89 of the 93 patients with primary biliary cirrhosis reacted with either the pyruvate dehydrogenase-E2 or the branched-chain α-keto acid dehydrogenase protein. None of the 124 control samples from healthy volunteers (n = 86) or patients with primary sclerosing cholangitis (n = 38) had significant reactivity. Our results indicate that the use of recombinant, cloned autoantigens provides a simple, accurate, and rapid method of quantifying and monitoring the levels of specific mitochondrial autoantibodies in the serum of patients with primary biliary cirrhosis.

Original languageEnglish (US)
Pages (from-to)1377-1380
Number of pages4
JournalNew England Journal of Medicine
Volume320
Issue number21
StatePublished - 1989

ASJC Scopus subject areas

  • Medicine(all)

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