Detection of Arbovirus Transmission via Sugar Feeding in a Laboratory Setting

Mary E. Danforth, William Reisen, Chris Barker

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Most species of mosquitoes consume sugar to survive and during sugar feeding can expectorate virus. Scientists have used this behavior to develop novel methods of mosquito control and arbovirus surveillance. In this study, we use sugar feeding and corresponding viral expectoration to develop an affordable method of monitoring individual mosquitoes for longitudinal data collection. Female Culex tarsalis Coquillett (Diptera: Culicidae) that consumed an infectious bloodmeal of West Nile virus were placed into separate containers and offered a sucrose-soaked cotton wick. Wicks were then collected daily and tested for virus with similar results to those from standard capillary tube method. This yielded a direct longitudinal estimate of the extrinsic incubation period, while using fewer mosquitoes. This approach could be used to further characterize variation in the amount and diversity of expectorated virus over the life span of individual mosquitoes.

Original languageEnglish (US)
Pages (from-to)1575-1579
Number of pages5
JournalJournal of Medical Entomology
Volume55
Issue number6
DOIs
StatePublished - Oct 25 2018

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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