Detection of anti-rotavirus antibody-producing cells in paraffin-embedded tissue sections.

S. L. Vonderfecht, Bennie Osburn

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An assay to detect anti-rotavirus antibody-producing cells was developed in rabbits. Sections of alcohol-fixed, paraffin-embedded tissues were used to make an immunofluorescent "sandwich" consisting of specific antibody-producing cells, a concentrated suspension of bovine rotavirus, and rhodamine-conjugated anti-rotavirus serum. Rhodamine-stained, anti-rotavirus antibody-producing cells could be simultaneously stained by a fluorescein-conjugated antiserum reactive to rabbit immunoglobulin.

Original languageEnglish (US)
Pages (from-to)356-359
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume43
Issue number2
StatePublished - Feb 1 1982
Externally publishedYes

Fingerprint

Antibody-Producing Cells
Rotavirus
Paraffin
alkanes
Anti-Idiotypic Antibodies
antibodies
Rhodamines
rabbits
cells
Rabbits
sandwiches
fluorescein
blood serum
Fluorescein
immunoglobulins
antiserum
Immunoglobulins
Immune Sera
Suspensions
alcohols

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Detection of anti-rotavirus antibody-producing cells in paraffin-embedded tissue sections. / Vonderfecht, S. L.; Osburn, Bennie.

In: American Journal of Veterinary Research, Vol. 43, No. 2, 01.02.1982, p. 356-359.

Research output: Contribution to journalArticle

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