An assay to detect anti-rotavirus antibody-producing cells was developed in rabbits. Sections of alcohol-fixed, paraffin-embedded tissues were used to make an immunofluorescent "sandwich" consisting of specific antibody-producing cells, a concentrated suspension of bovine rotavirus, and rhodamine-conjugated anti-rotavirus serum. Rhodamine-stained, anti-rotavirus antibody-producing cells could be simultaneously stained by a fluorescein-conjugated antiserum reactive to rabbit immunoglobulin.
|Original language||English (US)|
|Number of pages||4|
|Journal||American Journal of Veterinary Research|
|State||Published - Feb 1 1982|
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