Detection of acquired provirus sequences in mammary tumors from low-expressor, low-risk mice

B. W. Altrock, Robert Cardiff, J. P. Puma, J. K. Lund

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Murine mammary tumor virus (MuMTV) provirus sequences in the DNA from early-occurring (average age 10 mo) and late occurring (age >20 mo) tumors in BALB/cfC3H mice were analyzed by Eco RI restriction endonuclease mapping procedures. All early tumors were MuMTV antigen-positive mammary adenocarcinomas that contained the 0.92- and 4.0-kilo base( kb) exogenous C3H MuMTV-specific Pst I restriction endonuclease fragments. All but 1 of the late mammary adenocarcinomas had MuMTV antigens detected by peroxidase antiperoxidase staining, and all contained the 0.92- and 4.0-kb exogenous virus Pst I fragments. Three late nonmammary tumors lacked both MuMTV antigens and acquired provirus sequences. Greater numbers of MuMTV sequences were detected in both early and late-arising mammary tumors by Eco RI restriction endonuclease mapping than were detected in tissues from uninfected BALB/c mice. However, neither the number nor the location of MuMTV proviruses correlated with tumor latent period.

Original languageEnglish (US)
Pages (from-to)1037-1041
Number of pages5
JournalJournal of the National Cancer Institute
Volume68
Issue number6
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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