Detection of a respiratory coronavirus from tissues archived during a pneumonia epizootic in free-ranging pacific harbor seals Phoca vitulina richardsii

Hendrik H. Nollens, James F X Wellehan, Linda Archer, Linda J Lowenstine, Frances M D Gulland

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In June 2000, 21 adult harbor seals Phoca vitulina richardsii were found dead along a localized section of the central California coast. Necropsy of 5 fresh carcasses revealed pulmonary congestion, consolidation, and hemorrhage. Histopathological changes in lungs from 2 of these seals included a necrotizing lymphocytic and histocytic lobar pneumonia with intra-lesional bacteria. A coronavirus (CoV) was detected in archived tissues from 1 of the 5 seals via a degenerate PCR for nidoviral RNA-dependent RNA polymerase (RdRp), and was subsequently confirmed via specific PCR. Based on the partial RdRp sequence, the CoV was identified as a novel, divergent member of the CoV group 1a. The virus is tentatively named harbor seal coronavirus (HSCoV). The clinical significance of HSCoV and its involvement in the etiology of the epizootic pneumonia and deaths of the harbor seals is uncertain.

Original languageEnglish (US)
Pages (from-to)113-120
Number of pages8
JournalDiseases of Aquatic Organisms
Volume90
Issue number2
DOIs
StatePublished - Jun 11 2010

Fingerprint

Phoca vitulina
Coronavirinae
pneumonia
RNA
harbor
RNA-directed RNA polymerase
seals
lungs
etiology
congestion
consolidation
virus
hemorrhage
necropsy
bacterium
tissues
tissue
detection
coast
death

Keywords

  • Coronavirus
  • Harbor seal
  • Phoca vitulina richardsii
  • Pneumonia

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Detection of a respiratory coronavirus from tissues archived during a pneumonia epizootic in free-ranging pacific harbor seals Phoca vitulina richardsii. / Nollens, Hendrik H.; Wellehan, James F X; Archer, Linda; Lowenstine, Linda J; Gulland, Frances M D.

In: Diseases of Aquatic Organisms, Vol. 90, No. 2, 11.06.2010, p. 113-120.

Research output: Contribution to journalArticle

@article{09350adba5d3432f9135dbf025284168,
title = "Detection of a respiratory coronavirus from tissues archived during a pneumonia epizootic in free-ranging pacific harbor seals Phoca vitulina richardsii",
abstract = "In June 2000, 21 adult harbor seals Phoca vitulina richardsii were found dead along a localized section of the central California coast. Necropsy of 5 fresh carcasses revealed pulmonary congestion, consolidation, and hemorrhage. Histopathological changes in lungs from 2 of these seals included a necrotizing lymphocytic and histocytic lobar pneumonia with intra-lesional bacteria. A coronavirus (CoV) was detected in archived tissues from 1 of the 5 seals via a degenerate PCR for nidoviral RNA-dependent RNA polymerase (RdRp), and was subsequently confirmed via specific PCR. Based on the partial RdRp sequence, the CoV was identified as a novel, divergent member of the CoV group 1a. The virus is tentatively named harbor seal coronavirus (HSCoV). The clinical significance of HSCoV and its involvement in the etiology of the epizootic pneumonia and deaths of the harbor seals is uncertain.",
keywords = "Coronavirus, Harbor seal, Phoca vitulina richardsii, Pneumonia",
author = "Nollens, {Hendrik H.} and Wellehan, {James F X} and Linda Archer and Lowenstine, {Linda J} and Gulland, {Frances M D}",
year = "2010",
month = "6",
day = "11",
doi = "10.3354/dao02190",
language = "English (US)",
volume = "90",
pages = "113--120",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",
publisher = "Inter-Research",
number = "2",

}

TY - JOUR

T1 - Detection of a respiratory coronavirus from tissues archived during a pneumonia epizootic in free-ranging pacific harbor seals Phoca vitulina richardsii

AU - Nollens, Hendrik H.

AU - Wellehan, James F X

AU - Archer, Linda

AU - Lowenstine, Linda J

AU - Gulland, Frances M D

PY - 2010/6/11

Y1 - 2010/6/11

N2 - In June 2000, 21 adult harbor seals Phoca vitulina richardsii were found dead along a localized section of the central California coast. Necropsy of 5 fresh carcasses revealed pulmonary congestion, consolidation, and hemorrhage. Histopathological changes in lungs from 2 of these seals included a necrotizing lymphocytic and histocytic lobar pneumonia with intra-lesional bacteria. A coronavirus (CoV) was detected in archived tissues from 1 of the 5 seals via a degenerate PCR for nidoviral RNA-dependent RNA polymerase (RdRp), and was subsequently confirmed via specific PCR. Based on the partial RdRp sequence, the CoV was identified as a novel, divergent member of the CoV group 1a. The virus is tentatively named harbor seal coronavirus (HSCoV). The clinical significance of HSCoV and its involvement in the etiology of the epizootic pneumonia and deaths of the harbor seals is uncertain.

AB - In June 2000, 21 adult harbor seals Phoca vitulina richardsii were found dead along a localized section of the central California coast. Necropsy of 5 fresh carcasses revealed pulmonary congestion, consolidation, and hemorrhage. Histopathological changes in lungs from 2 of these seals included a necrotizing lymphocytic and histocytic lobar pneumonia with intra-lesional bacteria. A coronavirus (CoV) was detected in archived tissues from 1 of the 5 seals via a degenerate PCR for nidoviral RNA-dependent RNA polymerase (RdRp), and was subsequently confirmed via specific PCR. Based on the partial RdRp sequence, the CoV was identified as a novel, divergent member of the CoV group 1a. The virus is tentatively named harbor seal coronavirus (HSCoV). The clinical significance of HSCoV and its involvement in the etiology of the epizootic pneumonia and deaths of the harbor seals is uncertain.

KW - Coronavirus

KW - Harbor seal

KW - Phoca vitulina richardsii

KW - Pneumonia

UR - http://www.scopus.com/inward/record.url?scp=77954110896&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954110896&partnerID=8YFLogxK

U2 - 10.3354/dao02190

DO - 10.3354/dao02190

M3 - Article

VL - 90

SP - 113

EP - 120

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 2

ER -