Detection by polymerase chain reaction of Clostridium perfringens producing epsilon toxin in faeces and in gastrointestinal contents of goats

Francisco A Uzal, J. J. Plumb, L. L. Blackall, D. O'Boyle, W. R. Kelly

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A polymerase chain reaction (PCR) was used to identify the gene-encoding epsilon toxin production in Clostridium perfringens types B and D in faeces and in gastrointestinal contents of goats. The samples were cultured in thioglycollate broth and centrifuged. The upper layer of the pellet was used as a template for PCR, obviating the need for DNA extraction. This technique specifically differentiated Cl. perfringens types B and D from Cl. perfringens types A and C and from Escherichia coli. When used to identify Cl. perfringens type D in samples artificially spiked with the micro-organism, the PCR detected as few as 1.4 x 102 cfu g-1 of sample. Gastrointestinal contents and faeces were collected from 20 goats at slaughter and processed by PCR. Several positive results were obtained from the first five goats that were slaughtered and sampled a few days after their arrival at the abattoir, but only a few samples gave positive results during the following weeks, after the goats had been fed a concentrated ration containing monensin. A possible role of this drug in control of enterotoxaemia is suggested.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalLetters in Applied Microbiology
Volume23
Issue number1
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Water Science and Technology
  • Fluid Flow and Transfer Processes
  • Microbiology
  • Ecology

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