Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols

Janet V. Warg, Travis Clement, Emily R. Cornwell, Angela Cruz, Rodman G. Getchell, Cem Giray, Andrew E. Goodwin, Geoffrey H. Groocock, Mohamed Faisal, Robert Kim, Gwenn E. Merry, Nicholas B D Phelps, Monica M. Reising, Isaac Standish, Yan Zhang, Kathy L. Kurth

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays under consideration for deployment to multiple testing laboratories across the USA were evaluated for diagnostic sensitivity and specificity on tissue homogenates obtained from natural and experimental viral hemorrhagic septicemia (VHS)-infected fish. Estimates for diagnostic specificity using virus isolation as the reference method were similar between laboratories regardless of the assay. Diagnostic sensitivity estimates of 0.96 (95% CI: 0.95, 0.97) for Jonstrup et al. (2013)'s assay (J Fish Dis 36:9-23) exceeded the diagnostic sensitivity of 0.85 (95% CI: 0.83, 0.87) for Phelps et al. (2012)'s assay (J Aquat Anim Health 24:238-243). The Jonstrup rRT-PCR assay is robust as demonstrated by high sensitivity and specificity estimates across laboratories and can be used as a valuable tool for targeted surveillance and for testing of suspect VHSV samples.

Original languageEnglish (US)
Pages (from-to)15-22
Number of pages8
JournalDiseases of Aquatic Organisms
Volume111
Issue number1
DOIs
StatePublished - Aug 21 2014
Externally publishedYes

Fingerprint

Novirhabdovirus
Viral hemorrhagic septicemia virus
Real-Time Polymerase Chain Reaction
virus
diagnostic sensitivity
reverse transcriptase polymerase chain reaction
assay
Reverse Transcription
Viral Haemorrhagic Septicaemia
monitoring
Fishes
diagnostic specificity
assays
Sensitivity and Specificity
Polymerase Chain Reaction
polymerase chain reaction
Viruses
septicemia
fish
Health

Keywords

  • Diagnostic sensitivity
  • Diagnostic specificity
  • Real-time RT-PCR
  • Surveillance
  • VHSV

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

Cite this

Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols. / Warg, Janet V.; Clement, Travis; Cornwell, Emily R.; Cruz, Angela; Getchell, Rodman G.; Giray, Cem; Goodwin, Andrew E.; Groocock, Geoffrey H.; Faisal, Mohamed; Kim, Robert; Merry, Gwenn E.; Phelps, Nicholas B D; Reising, Monica M.; Standish, Isaac; Zhang, Yan; Kurth, Kathy L.

In: Diseases of Aquatic Organisms, Vol. 111, No. 1, 21.08.2014, p. 15-22.

Research output: Contribution to journalArticle

Warg, JV, Clement, T, Cornwell, ER, Cruz, A, Getchell, RG, Giray, C, Goodwin, AE, Groocock, GH, Faisal, M, Kim, R, Merry, GE, Phelps, NBD, Reising, MM, Standish, I, Zhang, Y & Kurth, KL 2014, 'Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols', Diseases of Aquatic Organisms, vol. 111, no. 1, pp. 15-22. https://doi.org/10.3354/dao02758
Warg, Janet V. ; Clement, Travis ; Cornwell, Emily R. ; Cruz, Angela ; Getchell, Rodman G. ; Giray, Cem ; Goodwin, Andrew E. ; Groocock, Geoffrey H. ; Faisal, Mohamed ; Kim, Robert ; Merry, Gwenn E. ; Phelps, Nicholas B D ; Reising, Monica M. ; Standish, Isaac ; Zhang, Yan ; Kurth, Kathy L. / Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols. In: Diseases of Aquatic Organisms. 2014 ; Vol. 111, No. 1. pp. 15-22.
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