Abstract
Introduction: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira spp. Leptospirosis is maintained in an environment due to chronic kidney infection of a wide variety of domestic, peridomestic and wild reservoir mammals. In this study the role of pigs in maintenance of leptospires on the Caribbean island of St. Kitts was investigated. Methodology: The condemned kidneys of 60 pigs slaughtered at a St. Kitts abattoir were screened by a quantitative-PCR for the presence of Leptospira spp. Positive samples were genotyped using a six-gene based multilocus sequence typing scheme. Results: Leptospiral DNA was detected in the kidneys of one of the 60 pigs. Multilocus sequence typing identified the infecting species to be L. interrogans. Conclusions: Detection of this zoonotic pathogen in the kidneys of a seemingly healthy pig raises concerns regarding the subclinical carriers of the disease among the island’s swine population.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 530-532 |
| Number of pages | 3 |
| Journal | Journal of Infection in Developing Countries |
| Volume | 9 |
| Issue number | 5 |
| DOIs | |
| State | Published - 2015 |
| Externally published | Yes |
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Keywords
- Genotyping
- Leptospira
- Leptospirosis
- Multi-locus sequence typing (MLST)
- Swine
ASJC Scopus subject areas
- Parasitology
- Microbiology
- Infectious Diseases
- Virology
Cite this
Detection and genotyping of Leptospira spp. From the kidneys of a seemingly healthy pig slaughtered for human consumption. / Verma, Ashutosh; Soto Martinez, Esteban; Illanes, Oscar; Ghosh, Souvik; Fuentealba, Carmen.
In: Journal of Infection in Developing Countries, Vol. 9, No. 5, 2015, p. 530-532.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection and genotyping of Leptospira spp. From the kidneys of a seemingly healthy pig slaughtered for human consumption
AU - Verma, Ashutosh
AU - Soto Martinez, Esteban
AU - Illanes, Oscar
AU - Ghosh, Souvik
AU - Fuentealba, Carmen
PY - 2015
Y1 - 2015
N2 - Introduction: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira spp. Leptospirosis is maintained in an environment due to chronic kidney infection of a wide variety of domestic, peridomestic and wild reservoir mammals. In this study the role of pigs in maintenance of leptospires on the Caribbean island of St. Kitts was investigated. Methodology: The condemned kidneys of 60 pigs slaughtered at a St. Kitts abattoir were screened by a quantitative-PCR for the presence of Leptospira spp. Positive samples were genotyped using a six-gene based multilocus sequence typing scheme. Results: Leptospiral DNA was detected in the kidneys of one of the 60 pigs. Multilocus sequence typing identified the infecting species to be L. interrogans. Conclusions: Detection of this zoonotic pathogen in the kidneys of a seemingly healthy pig raises concerns regarding the subclinical carriers of the disease among the island’s swine population.
AB - Introduction: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira spp. Leptospirosis is maintained in an environment due to chronic kidney infection of a wide variety of domestic, peridomestic and wild reservoir mammals. In this study the role of pigs in maintenance of leptospires on the Caribbean island of St. Kitts was investigated. Methodology: The condemned kidneys of 60 pigs slaughtered at a St. Kitts abattoir were screened by a quantitative-PCR for the presence of Leptospira spp. Positive samples were genotyped using a six-gene based multilocus sequence typing scheme. Results: Leptospiral DNA was detected in the kidneys of one of the 60 pigs. Multilocus sequence typing identified the infecting species to be L. interrogans. Conclusions: Detection of this zoonotic pathogen in the kidneys of a seemingly healthy pig raises concerns regarding the subclinical carriers of the disease among the island’s swine population.
KW - Genotyping
KW - Leptospira
KW - Leptospirosis
KW - Multi-locus sequence typing (MLST)
KW - Swine
UR - http://www.scopus.com/inward/record.url?scp=84929589732&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84929589732&partnerID=8YFLogxK
U2 - 10.3855/jidc.5727
DO - 10.3855/jidc.5727
M3 - Article
C2 - 25989174
AN - SCOPUS:84929589732
VL - 9
SP - 530
EP - 532
JO - Journal of Infection in Developing Countries
JF - Journal of Infection in Developing Countries
SN - 1972-2680
IS - 5
ER -