Detection and genotyping of Leptospira spp. From the kidneys of a seemingly healthy pig slaughtered for human consumption

Ashutosh Verma, Esteban Soto Martinez, Oscar Illanes, Souvik Ghosh, Carmen Fuentealba

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Introduction: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira spp. Leptospirosis is maintained in an environment due to chronic kidney infection of a wide variety of domestic, peridomestic and wild reservoir mammals. In this study the role of pigs in maintenance of leptospires on the Caribbean island of St. Kitts was investigated. Methodology: The condemned kidneys of 60 pigs slaughtered at a St. Kitts abattoir were screened by a quantitative-PCR for the presence of Leptospira spp. Positive samples were genotyped using a six-gene based multilocus sequence typing scheme. Results: Leptospiral DNA was detected in the kidneys of one of the 60 pigs. Multilocus sequence typing identified the infecting species to be L. interrogans. Conclusions: Detection of this zoonotic pathogen in the kidneys of a seemingly healthy pig raises concerns regarding the subclinical carriers of the disease among the island’s swine population.

Original languageEnglish (US)
Pages (from-to)530-532
Number of pages3
JournalJournal of Infection in Developing Countries
Volume9
Issue number5
DOIs
StatePublished - 2015
Externally publishedYes

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Keywords

  • Genotyping
  • Leptospira
  • Leptospirosis
  • Multi-locus sequence typing (MLST)
  • Swine

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Infectious Diseases
  • Virology

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