Abstract
Monoclonal antibodies can be used in sandwich enzyme-linked immunosorbent assay to measure viral antigens. Such an assay was developed to detect the core protein, p24, of human T-cell lymphotropic virus type III and lymphadenopathy-associated virus, etiologic agents of the acquired immunodeficiency syndrome (AIDS). Another AIDS-associated virus, AIDS-associated retrovirus type 2 (ARV-2) could not be detected in this assay because of the lower affinity of one of the monoclonal antibodies to ARV-2 p24. Detection of ARV-2 was accomplished with a monoclonal antibody-rabbit polyclonal antibody sandwich enzyme-linked immunosorbent assay. These two assays were used to efficiently detect AIDS-related viruses in lymphocyte cell cultures and to distinguish strains of the viruses.
Original language | English (US) |
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Pages (from-to) | 424-430 |
Number of pages | 7 |
Journal | Journal of Clinical Microbiology |
Volume | 24 |
Issue number | 3 |
State | Published - 1986 |
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)