Detection and classification of infectious bronchitis viruses isolated in Korea by dot-immunoblotting assay using monoclonal antibodies

Chang Seon Song, Jae Hong Kim, Youn Jeong Lee, Sun Joong Kim, Yoshihiro Izumiya, Yukinobu Tohya, Hyung Kwan Jang, Takeshi Mikami

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Dot-immunoblotting assay (DIA) using five monoclonal antibodies (MAbs) to infectious bronchitis virus (IBV) was used to detect and classify the viruses propagated in embryonated chicken eggs. Using a group-specific MAb 3F5, 10 reference strains and 12 Korean isolates of IBV were successfully detected by DIA, and the lowest virus titer of IBV detected by DIA was approximately less than 103.8 mean embryo infective dose/mi. For evaluating the diagnostic efficiency, DIA was compared with the conventional infectious bronchitis (IB) diagnostic method. IBV antigens in allantoic fluid from embryonated eggs inoculated with IB-suspected field samples were specifically detected by DIA within only one or two egg passages, whereas the conventional embryonated egg inoculation method required four to seven egg passages for confirming IBV infection. These results indicated that DIA could significantly reduce time and cost for IB diagnosis. For examining the possibility of classifying IBV by DIA, four strain-specific MAbs, 3A4, 2A3, 6F7, and 2C6, were used. According to the MAb reacting patterns to the IBV antigens, the 10 IBV reference strains were classified into six groups; seven strains belonged to three different groups, and the other three strains each belonged to an individual group. In the case of 12 Korean isolates of IBV, they were classified in six groups. Among the six groups, the MAb reacting patterns of three groups matched those of the IBV reference strains, but the others did not. These data suggest that at least three variant serotypes of IBV exist in Korea.

Original languageEnglish (US)
Pages (from-to)92-100
Number of pages9
JournalAvian Diseases
Volume42
Issue number1
StatePublished - Jan 1998
Externally publishedYes

Fingerprint

Infectious bronchitis virus
Korea
immunoblotting
Immunoblotting
Korean Peninsula
monoclonal antibodies
Monoclonal Antibodies
assays
infectious bronchitis
Bronchitis
Ovum
Eggs
allantoic fluid
antigens
Antigens
inoculation methods
chicken eggs
Virus Diseases
viral load
Viral Load

ASJC Scopus subject areas

  • Immunology
  • Cancer Research
  • veterinary(all)

Cite this

Detection and classification of infectious bronchitis viruses isolated in Korea by dot-immunoblotting assay using monoclonal antibodies. / Song, Chang Seon; Kim, Jae Hong; Lee, Youn Jeong; Kim, Sun Joong; Izumiya, Yoshihiro; Tohya, Yukinobu; Jang, Hyung Kwan; Mikami, Takeshi.

In: Avian Diseases, Vol. 42, No. 1, 01.1998, p. 92-100.

Research output: Contribution to journalArticle

Song, Chang Seon ; Kim, Jae Hong ; Lee, Youn Jeong ; Kim, Sun Joong ; Izumiya, Yoshihiro ; Tohya, Yukinobu ; Jang, Hyung Kwan ; Mikami, Takeshi. / Detection and classification of infectious bronchitis viruses isolated in Korea by dot-immunoblotting assay using monoclonal antibodies. In: Avian Diseases. 1998 ; Vol. 42, No. 1. pp. 92-100.
@article{7910755d24d44bd7bdc2e4240a81e344,
title = "Detection and classification of infectious bronchitis viruses isolated in Korea by dot-immunoblotting assay using monoclonal antibodies",
abstract = "Dot-immunoblotting assay (DIA) using five monoclonal antibodies (MAbs) to infectious bronchitis virus (IBV) was used to detect and classify the viruses propagated in embryonated chicken eggs. Using a group-specific MAb 3F5, 10 reference strains and 12 Korean isolates of IBV were successfully detected by DIA, and the lowest virus titer of IBV detected by DIA was approximately less than 103.8 mean embryo infective dose/mi. For evaluating the diagnostic efficiency, DIA was compared with the conventional infectious bronchitis (IB) diagnostic method. IBV antigens in allantoic fluid from embryonated eggs inoculated with IB-suspected field samples were specifically detected by DIA within only one or two egg passages, whereas the conventional embryonated egg inoculation method required four to seven egg passages for confirming IBV infection. These results indicated that DIA could significantly reduce time and cost for IB diagnosis. For examining the possibility of classifying IBV by DIA, four strain-specific MAbs, 3A4, 2A3, 6F7, and 2C6, were used. According to the MAb reacting patterns to the IBV antigens, the 10 IBV reference strains were classified into six groups; seven strains belonged to three different groups, and the other three strains each belonged to an individual group. In the case of 12 Korean isolates of IBV, they were classified in six groups. Among the six groups, the MAb reacting patterns of three groups matched those of the IBV reference strains, but the others did not. These data suggest that at least three variant serotypes of IBV exist in Korea.",
author = "Song, {Chang Seon} and Kim, {Jae Hong} and Lee, {Youn Jeong} and Kim, {Sun Joong} and Yoshihiro Izumiya and Yukinobu Tohya and Jang, {Hyung Kwan} and Takeshi Mikami",
year = "1998",
month = "1",
language = "English (US)",
volume = "42",
pages = "92--100",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "1",

}

TY - JOUR

T1 - Detection and classification of infectious bronchitis viruses isolated in Korea by dot-immunoblotting assay using monoclonal antibodies

AU - Song, Chang Seon

AU - Kim, Jae Hong

AU - Lee, Youn Jeong

AU - Kim, Sun Joong

AU - Izumiya, Yoshihiro

AU - Tohya, Yukinobu

AU - Jang, Hyung Kwan

AU - Mikami, Takeshi

PY - 1998/1

Y1 - 1998/1

N2 - Dot-immunoblotting assay (DIA) using five monoclonal antibodies (MAbs) to infectious bronchitis virus (IBV) was used to detect and classify the viruses propagated in embryonated chicken eggs. Using a group-specific MAb 3F5, 10 reference strains and 12 Korean isolates of IBV were successfully detected by DIA, and the lowest virus titer of IBV detected by DIA was approximately less than 103.8 mean embryo infective dose/mi. For evaluating the diagnostic efficiency, DIA was compared with the conventional infectious bronchitis (IB) diagnostic method. IBV antigens in allantoic fluid from embryonated eggs inoculated with IB-suspected field samples were specifically detected by DIA within only one or two egg passages, whereas the conventional embryonated egg inoculation method required four to seven egg passages for confirming IBV infection. These results indicated that DIA could significantly reduce time and cost for IB diagnosis. For examining the possibility of classifying IBV by DIA, four strain-specific MAbs, 3A4, 2A3, 6F7, and 2C6, were used. According to the MAb reacting patterns to the IBV antigens, the 10 IBV reference strains were classified into six groups; seven strains belonged to three different groups, and the other three strains each belonged to an individual group. In the case of 12 Korean isolates of IBV, they were classified in six groups. Among the six groups, the MAb reacting patterns of three groups matched those of the IBV reference strains, but the others did not. These data suggest that at least three variant serotypes of IBV exist in Korea.

AB - Dot-immunoblotting assay (DIA) using five monoclonal antibodies (MAbs) to infectious bronchitis virus (IBV) was used to detect and classify the viruses propagated in embryonated chicken eggs. Using a group-specific MAb 3F5, 10 reference strains and 12 Korean isolates of IBV were successfully detected by DIA, and the lowest virus titer of IBV detected by DIA was approximately less than 103.8 mean embryo infective dose/mi. For evaluating the diagnostic efficiency, DIA was compared with the conventional infectious bronchitis (IB) diagnostic method. IBV antigens in allantoic fluid from embryonated eggs inoculated with IB-suspected field samples were specifically detected by DIA within only one or two egg passages, whereas the conventional embryonated egg inoculation method required four to seven egg passages for confirming IBV infection. These results indicated that DIA could significantly reduce time and cost for IB diagnosis. For examining the possibility of classifying IBV by DIA, four strain-specific MAbs, 3A4, 2A3, 6F7, and 2C6, were used. According to the MAb reacting patterns to the IBV antigens, the 10 IBV reference strains were classified into six groups; seven strains belonged to three different groups, and the other three strains each belonged to an individual group. In the case of 12 Korean isolates of IBV, they were classified in six groups. Among the six groups, the MAb reacting patterns of three groups matched those of the IBV reference strains, but the others did not. These data suggest that at least three variant serotypes of IBV exist in Korea.

UR - http://www.scopus.com/inward/record.url?scp=0031922209&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031922209&partnerID=8YFLogxK

M3 - Article

C2 - 9533085

AN - SCOPUS:0031922209

VL - 42

SP - 92

EP - 100

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 1

ER -