Detecting opioid metabolites in exhaled breath condensate (EBC)

Eva Borras, Andy Cheng, Ted Wun, Kristen L. Reese, Matthias Frank, Michael Schivo, Cristina E. Davis

Research output: Contribution to journalArticle

Abstract

Exhaled breath condensate (EBC) collection provides a promising matrix for bioanalysis of endogenous biomarkers of health and also for exogenous compounds like drugs. There is little information regarding drugs and their metabolites contained in breath, as well as their pharmacokinetics. In this present work, we use a simple and non-invasive technique to collect EBC from chronic pain patients using different analgesic opioid drugs to manage pain. Six patients received continuous infusion of morphine and hydromorphone intravenously (IV), together with other analgesic drugs (IV and orally). Repeated sampling of serum and EBC was done at two time points separated by 90 min. The EBC was collected using a glass tube surrounded by dry ice, and an ethanol solvent wash of the glass was performed after EBC extraction to retrieve the apolar compounds stuck to the glass surface. All samples were analyzed with liquid chromatography coupled to mass spectrometry (LC-MS/MS) to identify possible metabolites present in the sample, and to quantify the drugs being used. Several metabolites, such as normorphine (norM), norhydromorphone (norHM) and dihydromorphone (diHM) were detected in both fractions, while hydromorphone 3-glucuronide (HM 3G) was only detected in the solvent rinse fraction. Results were correlated to explain the pharmacokinetics of the main drugs administered. This pilot study presented promising correlations between drug concentrations in blood and breath at different time points for norM, norHM and HM 3G.

Original languageEnglish (US)
Number of pages1
JournalJournal of breath research
Volume13
Issue number4
DOIs
StatePublished - Oct 3 2019

Fingerprint

Opioid Analgesics
Glass
Pharmaceutical Preparations
Analgesics
Pharmacokinetics
Dry Ice
Hydromorphone
Liquid Chromatography
Chronic Pain
Morphine
Mass Spectrometry
Ethanol
Biomarkers
Pain
Health
Serum
norhydromorphone
normorphine
hydromorphone-3-glucuronide

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Detecting opioid metabolites in exhaled breath condensate (EBC). / Borras, Eva; Cheng, Andy; Wun, Ted; Reese, Kristen L.; Frank, Matthias; Schivo, Michael; Davis, Cristina E.

In: Journal of breath research, Vol. 13, No. 4, 03.10.2019.

Research output: Contribution to journalArticle

@article{7b04ea1a608c447f8780fc12446f5829,
title = "Detecting opioid metabolites in exhaled breath condensate (EBC)",
abstract = "Exhaled breath condensate (EBC) collection provides a promising matrix for bioanalysis of endogenous biomarkers of health and also for exogenous compounds like drugs. There is little information regarding drugs and their metabolites contained in breath, as well as their pharmacokinetics. In this present work, we use a simple and non-invasive technique to collect EBC from chronic pain patients using different analgesic opioid drugs to manage pain. Six patients received continuous infusion of morphine and hydromorphone intravenously (IV), together with other analgesic drugs (IV and orally). Repeated sampling of serum and EBC was done at two time points separated by 90 min. The EBC was collected using a glass tube surrounded by dry ice, and an ethanol solvent wash of the glass was performed after EBC extraction to retrieve the apolar compounds stuck to the glass surface. All samples were analyzed with liquid chromatography coupled to mass spectrometry (LC-MS/MS) to identify possible metabolites present in the sample, and to quantify the drugs being used. Several metabolites, such as normorphine (norM), norhydromorphone (norHM) and dihydromorphone (diHM) were detected in both fractions, while hydromorphone 3-glucuronide (HM 3G) was only detected in the solvent rinse fraction. Results were correlated to explain the pharmacokinetics of the main drugs administered. This pilot study presented promising correlations between drug concentrations in blood and breath at different time points for norM, norHM and HM 3G.",
author = "Eva Borras and Andy Cheng and Ted Wun and Reese, {Kristen L.} and Matthias Frank and Michael Schivo and Davis, {Cristina E.}",
year = "2019",
month = "10",
day = "3",
doi = "10.1088/1752-7163/ab35fd",
language = "English (US)",
volume = "13",
journal = "Journal of Breath Research",
issn = "1752-7155",
publisher = "IOP Publishing Ltd.",
number = "4",

}

TY - JOUR

T1 - Detecting opioid metabolites in exhaled breath condensate (EBC)

AU - Borras, Eva

AU - Cheng, Andy

AU - Wun, Ted

AU - Reese, Kristen L.

AU - Frank, Matthias

AU - Schivo, Michael

AU - Davis, Cristina E.

PY - 2019/10/3

Y1 - 2019/10/3

N2 - Exhaled breath condensate (EBC) collection provides a promising matrix for bioanalysis of endogenous biomarkers of health and also for exogenous compounds like drugs. There is little information regarding drugs and their metabolites contained in breath, as well as their pharmacokinetics. In this present work, we use a simple and non-invasive technique to collect EBC from chronic pain patients using different analgesic opioid drugs to manage pain. Six patients received continuous infusion of morphine and hydromorphone intravenously (IV), together with other analgesic drugs (IV and orally). Repeated sampling of serum and EBC was done at two time points separated by 90 min. The EBC was collected using a glass tube surrounded by dry ice, and an ethanol solvent wash of the glass was performed after EBC extraction to retrieve the apolar compounds stuck to the glass surface. All samples were analyzed with liquid chromatography coupled to mass spectrometry (LC-MS/MS) to identify possible metabolites present in the sample, and to quantify the drugs being used. Several metabolites, such as normorphine (norM), norhydromorphone (norHM) and dihydromorphone (diHM) were detected in both fractions, while hydromorphone 3-glucuronide (HM 3G) was only detected in the solvent rinse fraction. Results were correlated to explain the pharmacokinetics of the main drugs administered. This pilot study presented promising correlations between drug concentrations in blood and breath at different time points for norM, norHM and HM 3G.

AB - Exhaled breath condensate (EBC) collection provides a promising matrix for bioanalysis of endogenous biomarkers of health and also for exogenous compounds like drugs. There is little information regarding drugs and their metabolites contained in breath, as well as their pharmacokinetics. In this present work, we use a simple and non-invasive technique to collect EBC from chronic pain patients using different analgesic opioid drugs to manage pain. Six patients received continuous infusion of morphine and hydromorphone intravenously (IV), together with other analgesic drugs (IV and orally). Repeated sampling of serum and EBC was done at two time points separated by 90 min. The EBC was collected using a glass tube surrounded by dry ice, and an ethanol solvent wash of the glass was performed after EBC extraction to retrieve the apolar compounds stuck to the glass surface. All samples were analyzed with liquid chromatography coupled to mass spectrometry (LC-MS/MS) to identify possible metabolites present in the sample, and to quantify the drugs being used. Several metabolites, such as normorphine (norM), norhydromorphone (norHM) and dihydromorphone (diHM) were detected in both fractions, while hydromorphone 3-glucuronide (HM 3G) was only detected in the solvent rinse fraction. Results were correlated to explain the pharmacokinetics of the main drugs administered. This pilot study presented promising correlations between drug concentrations in blood and breath at different time points for norM, norHM and HM 3G.

UR - http://www.scopus.com/inward/record.url?scp=85072904689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072904689&partnerID=8YFLogxK

U2 - 10.1088/1752-7163/ab35fd

DO - 10.1088/1752-7163/ab35fd

M3 - Article

C2 - 31349234

AN - SCOPUS:85072904689

VL - 13

JO - Journal of Breath Research

JF - Journal of Breath Research

SN - 1752-7155

IS - 4

ER -