Desmoid cell motility is induced in vitro by rhEGF

David E. Joyner, Sylvia H. Trang, Timothy A. Damron, Albert J. Aboulafia, Judd E. Cummings, R Randall

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Desmoid tumors are benign but locally invasive myofibroblastic lesions that arise predominantly in the abdominal wall or shoulderand are prone to aggressive local recurrences. A perceived association between desmoid activity and the expression of growth factors during pregnancy or following trauma suggests a cause-and-effect relationship between growth factor stimulation and desmoid invasiveness. We used Boyden Chambers to quantify cell motility in order to determine the effect of growth factor stimulation on desmoid cell migration. Desmoid cell cultures were treated under serum-free conditions with epidermal growth factor (rhEGF) or transforming growth factor alpha (rhTGFα). Additional cell cultures were pretreated under serum-free conditions with the EGF receptor (EGFR) inhibitor AG1478, alone or in combination with the TGFβ1 receptor inhibitor SB431542, and then stimulated with growth factor prior to being assayed for cell motility. The experiments demonstrated a direct dose-dependent relationship between rhEGF stimulation and desmoid motility. In contrast, rhTGFα was less effective at inducing cell migration. rhEGF-induced cell migration could be diminished, but not reduced to control levels, by inhibiting EGFR. When EGF and TGFβ1 receptors were inhibited simultaneously, the level of rhEGF-induced cell migration was reduced significantly beyond the level of cell migration generated by inhibition of EGFR alone.

Original languageEnglish (US)
Pages (from-to)1258-1262
Number of pages5
JournalJournal of Orthopaedic Research
Volume27
Issue number9
DOIs
StatePublished - Sep 1 2009
Externally publishedYes

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Aggressive Fibromatosis
Cell Movement
Intercellular Signaling Peptides and Proteins
Epidermal Growth Factor Receptor
Transforming Growth Factor alpha
Cell Culture Techniques
Cell Migration Inhibition
Abdominal Wall
Serum
Epidermal Growth Factor
In Vitro Techniques
Recurrence
Pregnancy
Wounds and Injuries
Neoplasms

Keywords

  • Cell motility
  • Desmoid tumor
  • Growth factors
  • In vitro

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Joyner, D. E., Trang, S. H., Damron, T. A., Aboulafia, A. J., Cummings, J. E., & Randall, R. (2009). Desmoid cell motility is induced in vitro by rhEGF. Journal of Orthopaedic Research, 27(9), 1258-1262. https://doi.org/10.1002/jor.20865

Desmoid cell motility is induced in vitro by rhEGF. / Joyner, David E.; Trang, Sylvia H.; Damron, Timothy A.; Aboulafia, Albert J.; Cummings, Judd E.; Randall, R.

In: Journal of Orthopaedic Research, Vol. 27, No. 9, 01.09.2009, p. 1258-1262.

Research output: Contribution to journalArticle

Joyner, DE, Trang, SH, Damron, TA, Aboulafia, AJ, Cummings, JE & Randall, R 2009, 'Desmoid cell motility is induced in vitro by rhEGF', Journal of Orthopaedic Research, vol. 27, no. 9, pp. 1258-1262. https://doi.org/10.1002/jor.20865
Joyner DE, Trang SH, Damron TA, Aboulafia AJ, Cummings JE, Randall R. Desmoid cell motility is induced in vitro by rhEGF. Journal of Orthopaedic Research. 2009 Sep 1;27(9):1258-1262. https://doi.org/10.1002/jor.20865
Joyner, David E. ; Trang, Sylvia H. ; Damron, Timothy A. ; Aboulafia, Albert J. ; Cummings, Judd E. ; Randall, R. / Desmoid cell motility is induced in vitro by rhEGF. In: Journal of Orthopaedic Research. 2009 ; Vol. 27, No. 9. pp. 1258-1262.
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