Designing electroactive biointerface for spatiotemporal control of cell attachment and release

Sunny Shah, He Zhu, Jun Yan, Stanislav Verkhoturov, Alexander Revzin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we demonstrate the use of individually addressable microelectrodes for cell sorting and cell micropatterning applications. Microelectrodes were modified with cell adhesive or non-adhesive molecules and then electrically stimulated to selectively adsorb or desorb proteins and/or mammalian cells. The switching of the surface properties was achieved by the electrochemical desorption of protein-functionalized thiols and poly(ethylene glycol) PEG silane from gold and indium tin oxide (ITO) electrodes respectively. The thiol surfaces were modified with anti-CD4 antibodies and used to capture T-cells. Upon electrical activation of the microelectrodes, both the antibodies and the T-cells were removed from the specific locations on the substrate. In addition, ITO electrodes were modified with cellresistive PEG silane which was later electrochemically desorbed to make the surface adhesive to proteins or cells. This technique was employed to pattern two different cell types on the same substrate.

Original languageEnglish (US)
Title of host publicationECS Transactions
Pages17-25
Number of pages9
Volume13
Edition22
DOIs
StatePublished - 2008
EventElectrochemical Nano/Biosensors - 213th Meeting of the Electrochemical Society - Phoenix, AZ, United States
Duration: May 18 2008May 23 2009

Other

OtherElectrochemical Nano/Biosensors - 213th Meeting of the Electrochemical Society
CountryUnited States
CityPhoenix, AZ
Period5/18/085/23/09

Fingerprint

Microelectrodes
Polyethylene glycols
T-cells
Tin oxides
Proteins
Silanes
Antibodies
Indium
Adhesives
Cells
Electrodes
Substrates
Sorting
Surface properties
Desorption
Gold
Chemical activation
Molecules

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Shah, S., Zhu, H., Yan, J., Verkhoturov, S., & Revzin, A. (2008). Designing electroactive biointerface for spatiotemporal control of cell attachment and release. In ECS Transactions (22 ed., Vol. 13, pp. 17-25) https://doi.org/10.1149/1.3005399

Designing electroactive biointerface for spatiotemporal control of cell attachment and release. / Shah, Sunny; Zhu, He; Yan, Jun; Verkhoturov, Stanislav; Revzin, Alexander.

ECS Transactions. Vol. 13 22. ed. 2008. p. 17-25.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shah, S, Zhu, H, Yan, J, Verkhoturov, S & Revzin, A 2008, Designing electroactive biointerface for spatiotemporal control of cell attachment and release. in ECS Transactions. 22 edn, vol. 13, pp. 17-25, Electrochemical Nano/Biosensors - 213th Meeting of the Electrochemical Society, Phoenix, AZ, United States, 5/18/08. https://doi.org/10.1149/1.3005399
Shah S, Zhu H, Yan J, Verkhoturov S, Revzin A. Designing electroactive biointerface for spatiotemporal control of cell attachment and release. In ECS Transactions. 22 ed. Vol. 13. 2008. p. 17-25 https://doi.org/10.1149/1.3005399
Shah, Sunny ; Zhu, He ; Yan, Jun ; Verkhoturov, Stanislav ; Revzin, Alexander. / Designing electroactive biointerface for spatiotemporal control of cell attachment and release. ECS Transactions. Vol. 13 22. ed. 2008. pp. 17-25
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