Design of novel sequence-specific DNA-binding proteins

David Segal, Carlos F. Barbas

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


The design and selection of DNA-binding proteins or individual domains capable of novel sequence recognition continues to make great strides. Recent studies have also highlighted the role of the non-DNA-contacting portions of the protein and the optimal assembly of the domains. For the first time, it appears that it is possible to produce proteins capable of targeting any gene with an 18 base pair recognition domain. A variety of applications are being explored, such as targeted transcriptional regulation, recombination and viral integration. These proteins will probably find diverse applications in gene therapy, functional genomics, and agriculture.

Original languageEnglish (US)
Pages (from-to)34-39
Number of pages6
JournalCurrent Opinion in Chemical Biology
Issue number1
StatePublished - Feb 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry


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