Design, construction, and application of transcription activation-like effectors

Peter Deng, Sakereh Carter, Kyle Fink

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Transcription activator-like effectors (TALEs) are modular proteins derived from the plant Xanthomonas sp. pathogen that can be designed to target unique DNA sequences following a simple cipher. Customized TALE proteins can be used in a variety of molecular applications that include gene editing and transcriptional modulation. Presently, we provide a brief primer on the design and construction of TALEs. TALE proteins can be fused to a variety of different effector domains that alter the function of the TALE upon binding. This flexibility of TALE design and downstream effect may offer therapeutic applications that are discussed in this section. Finally, we provide a future perspective on TALE technology and what challenges remain for successful translation of gene-editing strategies to the clinic.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages47-58
Number of pages12
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1937
ISSN (Print)1064-3745

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Keywords

  • Artificial transcription factor
  • Chromatin remodeling
  • DNA binding
  • Gene editing
  • Huntington’s disease
  • Nucleases
  • TALEs

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Deng, P., Carter, S., & Fink, K. (2019). Design, construction, and application of transcription activation-like effectors. In Methods in Molecular Biology (pp. 47-58). (Methods in Molecular Biology; Vol. 1937). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9065-8_3