TY - JOUR
T1 - Design and fabrication of a highly sensitive and naked-eye distinguishable colorimetric biosensor for chloramphenicol detection by using ELISA on nanofibrous membranes
AU - Zhao, Cunyi
AU - Si, Yang
AU - Pan, Bofeng
AU - Taha, Ameer Y.
AU - Pan, Tingrui
AU - Sun, Gang
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Enzyme-linked immunoassay (ELISA) is highly specific and selective towards target molecules and is convenient for on-site detection. However, in many cases, lack of high sensitivity makes it hard to reveal a significant colorimetric signal for detecting a trace amount of target molecules. Thus, analytical instruments are required for detection, which limits the application of ELISA for on-site detection. In the present study, a highly sensitive and naked-eyed detectable colorimetric biosensor for chloramphenicol (CAP) was prepared by incorporating ELISA onto surfaces of microporous and nanofibrous membranes. The high specific surface areas of the nanofibers significantly increased the number of antibodies covalently linked onto the fiber surfaces and binding capacity of the sensor with antigens present in a sample. With such an integration, the sensitivity of the ELISA sensor was dramatically increased, and a trace number of targets could reveal a naked-eye detectable color. The immunoassay sensor exhibited a significant naked-eye distinguishable color to chloramphenicol (CAP) at 0.3 ng/mL. The successful design and fabrication of the nanofibrous membrane immunoassay sensor provide new paths towards the development of on-site inspection sensors without the assistance from any instrument.
AB - Enzyme-linked immunoassay (ELISA) is highly specific and selective towards target molecules and is convenient for on-site detection. However, in many cases, lack of high sensitivity makes it hard to reveal a significant colorimetric signal for detecting a trace amount of target molecules. Thus, analytical instruments are required for detection, which limits the application of ELISA for on-site detection. In the present study, a highly sensitive and naked-eyed detectable colorimetric biosensor for chloramphenicol (CAP) was prepared by incorporating ELISA onto surfaces of microporous and nanofibrous membranes. The high specific surface areas of the nanofibers significantly increased the number of antibodies covalently linked onto the fiber surfaces and binding capacity of the sensor with antigens present in a sample. With such an integration, the sensitivity of the ELISA sensor was dramatically increased, and a trace number of targets could reveal a naked-eye detectable color. The immunoassay sensor exhibited a significant naked-eye distinguishable color to chloramphenicol (CAP) at 0.3 ng/mL. The successful design and fabrication of the nanofibrous membrane immunoassay sensor provide new paths towards the development of on-site inspection sensors without the assistance from any instrument.
KW - Chloramphenicol
KW - Colorimetric sensor
KW - Enzyme-linked immunoassay
KW - Enzyme-linked immunosorbent assay
KW - Naked-eye distinction
KW - Nanofibrous membrane
UR - http://www.scopus.com/inward/record.url?scp=85083634248&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85083634248&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2020.121054
DO - 10.1016/j.talanta.2020.121054
M3 - Article
C2 - 32498843
AN - SCOPUS:85083634248
VL - 217
JO - Talanta
JF - Talanta
SN - 0039-9140
M1 - 121054
ER -