Design and evaluation of a device for fast multispectral time-resolved fluorescence spectroscopy and imaging

Diego R. Yankelevich, Dinglong Ma, Jing Liu, Yang Sun, Yinghua Sun, Julien Bec, Daniel S. Elson, Laura Marcu

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

The application of time-resolved fluorescence spectroscopy (TRFS) to in vivo tissue diagnosis requires a method for fast acquisition of fluorescence decay profiles in multiple spectral bands. This study focusses on development of a clinically compatible fiber-optic based multispectral TRFS (ms-TRFS) system together with validation of its accuracy and precision for fluorescence lifetime measurements. It also presents the expansion of this technique into an imaging spectroscopy method. A tandem array of dichroic beamsplitters and filters was used to record TRFS decay profiles at four distinct spectral bands where biological tissue typically presents fluorescence emission maxima, namely, 390, 452, 542, and 629 nm. Each emission channel was temporally separated by using transmission delays through 200 μ.

Original languageEnglish (US)
Article number034303
JournalReview of Scientific Instruments
Volume85
Issue number3
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Instrumentation

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    Yankelevich, D. R., Ma, D., Liu, J., Sun, Y., Sun, Y., Bec, J., Elson, D. S., & Marcu, L. (2014). Design and evaluation of a device for fast multispectral time-resolved fluorescence spectroscopy and imaging. Review of Scientific Instruments, 85(3), [034303]. https://doi.org/10.1063/1.4869037