Abstract
This study was undertaken to investigate the binding activity of Streptococcus suis serotype 2 to albumin. Using flow cytometry we observed a binding activity of S. suis to albumin for virulent as well as for avirulent isolates. Western immunoblots analysis revealed that a 39-kDa S. suis protein was responsible, at least in part, for this binding activity. This protein showed high N-terminal homology (95.6% for the first 23 residues) with a group A Streptococcus glyceraldehyde-3-phosphate dehydrogenase. Furthermore, the addition of albumin to the culture broth resulted in an increase in the virulence of S. suis strains in mice. These results suggest that an interaction with albumin could play a role in the pathogenesis of S. suis serotype 2 infections.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 245-250 |
| Number of pages | 6 |
| Journal | FEMS Microbiology Letters |
| Volume | 147 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 15 1997 |
| Externally published | Yes |
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Keywords
- albumin
- binding protein
- Streptococcus suis
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics
Cite this
Description of an albumin binding activity for Streptococcus suis serotype 2. / Quessy, Sylvain; Busque, Philippe; Higgins, Robert; Jacques, Mario; Dubreuil, J. Daniel.
In: FEMS Microbiology Letters, Vol. 147, No. 2, 15.02.1997, p. 245-250.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Description of an albumin binding activity for Streptococcus suis serotype 2
AU - Quessy, Sylvain
AU - Busque, Philippe
AU - Higgins, Robert
AU - Jacques, Mario
AU - Dubreuil, J. Daniel
PY - 1997/2/15
Y1 - 1997/2/15
N2 - This study was undertaken to investigate the binding activity of Streptococcus suis serotype 2 to albumin. Using flow cytometry we observed a binding activity of S. suis to albumin for virulent as well as for avirulent isolates. Western immunoblots analysis revealed that a 39-kDa S. suis protein was responsible, at least in part, for this binding activity. This protein showed high N-terminal homology (95.6% for the first 23 residues) with a group A Streptococcus glyceraldehyde-3-phosphate dehydrogenase. Furthermore, the addition of albumin to the culture broth resulted in an increase in the virulence of S. suis strains in mice. These results suggest that an interaction with albumin could play a role in the pathogenesis of S. suis serotype 2 infections.
AB - This study was undertaken to investigate the binding activity of Streptococcus suis serotype 2 to albumin. Using flow cytometry we observed a binding activity of S. suis to albumin for virulent as well as for avirulent isolates. Western immunoblots analysis revealed that a 39-kDa S. suis protein was responsible, at least in part, for this binding activity. This protein showed high N-terminal homology (95.6% for the first 23 residues) with a group A Streptococcus glyceraldehyde-3-phosphate dehydrogenase. Furthermore, the addition of albumin to the culture broth resulted in an increase in the virulence of S. suis strains in mice. These results suggest that an interaction with albumin could play a role in the pathogenesis of S. suis serotype 2 infections.
KW - albumin
KW - binding protein
KW - Streptococcus suis
UR - http://www.scopus.com/inward/record.url?scp=0031056274&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031056274&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(96)00534-4
DO - 10.1016/S0378-1097(96)00534-4
M3 - Article
C2 - 9119200
AN - SCOPUS:0031056274
VL - 147
SP - 245
EP - 250
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -