Description of a new Neospora species (Protozoa

Apicomplexa: Sarcocystidae)

Antoinette E. Marsh, Bradd C. Barr, Andrea E. Packham, Patricia A Conrad

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Neospora hughesi n. sp. was isolated from the central nervous system tissue of an adult equine (Equus caballus) from California. The tachyzoites are crescent-shaped, approximately 2 x 5 μm (1.8-3.0 x 4.0-7.0 μm), with characteristic apical complex structures consisting of an anterior polar ring, conoid, numerous rhoptries filled with a uniform electron-dense material, and 22 microtubules extending posteriorly from the polar ring. Comparison of N. hughesi to canine and bovine Neospora caninum isolates showed phenotypic differences in immunoreactive proteins. Molecular analysis of the small subunit ribosomal RNA gene revealed no differences in the nucleotide sequence between N. hughesi and N. caninum isolates examined. However, the internal transcribed spacer 1 region revealed 7 nucleotide base differences between N. hughesi and N. caninum isolates (CN1 and BPA1) analyzed in this study. The existence of nucleotide base differences in the internal transcribed spacer regions suggests that this region may be a genetic marker for discriminating species within the genus Neospora. The ultrastructural, antigenic, and molecular data support distinction of N. hughesi as a new species, separate from N. caninum, the only recognized species in this genus.

Original languageEnglish (US)
Pages (from-to)983-991
Number of pages9
JournalJournal of Parasitology
Volume84
Issue number5
DOIs
StatePublished - Oct 1998

Fingerprint

Neospora hughesi
Sarcocystidae
Neospora
Apicomplexa
Neospora caninum
Protozoa
new species
molecular analysis
internal transcribed spacers
genetic marker
nervous system
Horses
nucleotides
RNA
horses
Nucleotides
tachyzoites
Nerve Tissue
electron
protein

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Description of a new Neospora species (Protozoa : Apicomplexa: Sarcocystidae). / Marsh, Antoinette E.; Barr, Bradd C.; Packham, Andrea E.; Conrad, Patricia A.

In: Journal of Parasitology, Vol. 84, No. 5, 10.1998, p. 983-991.

Research output: Contribution to journalArticle

Marsh, Antoinette E. ; Barr, Bradd C. ; Packham, Andrea E. ; Conrad, Patricia A. / Description of a new Neospora species (Protozoa : Apicomplexa: Sarcocystidae). In: Journal of Parasitology. 1998 ; Vol. 84, No. 5. pp. 983-991.
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