Description of a closed window technique for in vivo study of the feline basilar artery

Gerrit J. Bouma, Joseph E. Levasseur, Jan Paul Muizelaar, Robin G. Wiatt, Hermes A. Kontos

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recent interest in the regulatory functions of large cerebral arteries has led to many studies addressing the specific reactivity of these vessels. Current data originate mainly from in vitro experiments, as in vivo studies of larger intracranial cerebral arteries have been cumbersome so far due to the lack of a suitable animal model. We provide a detailed technical description of a closed transclival window method for in vivo study of the basilar artery in cats. We present our experience with this preparation in 29 animals, which shows that the technique is feasible and allows repeated, accurate, and reproducible measurements of the basilar artery, although possible depressive effects of the anesthesia on vascular reactivity have to be taken into account. With hyperventilation, the basilar artery constricted by 12.2±7.6% of the baseline diameter. The cerebral blood flow response to hypocapnia with this preparation was 2.0±0.4%/torr PaCO2. An exudative clouding of the window occurred in some cats but had no apparent effect on vascular reactivity. We also discuss possible pitfalls in the surgical preparation.

Original languageEnglish (US)
Pages (from-to)522-526
Number of pages5
JournalStroke
Volume22
Issue number4
StatePublished - Apr 1991
Externally publishedYes

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Basilar Artery
Felidae
Cerebral Arteries
Blood Vessels
Cerebrovascular Circulation
Cats
Hypocapnia
Hyperventilation
Anesthesia
Animal Models

Keywords

  • Animal models
  • Basilar artery
  • Cats

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Bouma, G. J., Levasseur, J. E., Muizelaar, J. P., Wiatt, R. G., & Kontos, H. A. (1991). Description of a closed window technique for in vivo study of the feline basilar artery. Stroke, 22(4), 522-526.

Description of a closed window technique for in vivo study of the feline basilar artery. / Bouma, Gerrit J.; Levasseur, Joseph E.; Muizelaar, Jan Paul; Wiatt, Robin G.; Kontos, Hermes A.

In: Stroke, Vol. 22, No. 4, 04.1991, p. 522-526.

Research output: Contribution to journalArticle

Bouma, GJ, Levasseur, JE, Muizelaar, JP, Wiatt, RG & Kontos, HA 1991, 'Description of a closed window technique for in vivo study of the feline basilar artery', Stroke, vol. 22, no. 4, pp. 522-526.
Bouma GJ, Levasseur JE, Muizelaar JP, Wiatt RG, Kontos HA. Description of a closed window technique for in vivo study of the feline basilar artery. Stroke. 1991 Apr;22(4):522-526.
Bouma, Gerrit J. ; Levasseur, Joseph E. ; Muizelaar, Jan Paul ; Wiatt, Robin G. ; Kontos, Hermes A. / Description of a closed window technique for in vivo study of the feline basilar artery. In: Stroke. 1991 ; Vol. 22, No. 4. pp. 522-526.
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