Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains

Madhu S. Budamagunta; John F. Hess; Paul G FitzGerald; John C Voss

doi:10.1007/s12013-007-0035-4

Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains

Madhu S. Budamagunta, John F. Hess, Paul G FitzGerald, John C Voss

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.

Original language	English (US)
Pages (from-to)	45-53
Number of pages	9
Journal	Cell Biochemistry and Biophysics
Volume	48
Issue number	1
DOIs	https://doi.org/10.1007/s12013-007-0035-4
State	Published - May 2007

Keywords

EPR (ESR) spectroscopy
Intermediate filaments
Site-directed spin labeling
Vimentin

ASJC Scopus subject areas

Cell Biology
Clinical Biochemistry
Biophysics

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keywords = "EPR (ESR) spectroscopy, Intermediate filaments, Site-directed spin labeling, Vimentin",

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AU - Hess, John F.

AU - FitzGerald, Paul G

AU - Voss, John C

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AB - In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.

KW - EPR (ESR) spectroscopy

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KW - Site-directed spin labeling

KW - Vimentin

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Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains

Abstract

Keywords

ASJC Scopus subject areas

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