Abstract
In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 45-53 |
| Number of pages | 9 |
| Journal | Cell Biochemistry and Biophysics |
| Volume | 48 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 2007 |
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Keywords
- EPR (ESR) spectroscopy
- Intermediate filaments
- Site-directed spin labeling
- Vimentin
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Biophysics
Cite this
Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains. / Budamagunta, Madhu S.; Hess, John F.; FitzGerald, Paul G; Voss, John C.
In: Cell Biochemistry and Biophysics, Vol. 48, No. 1, 05.2007, p. 45-53.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains
AU - Budamagunta, Madhu S.
AU - Hess, John F.
AU - FitzGerald, Paul G
AU - Voss, John C
PY - 2007/5
Y1 - 2007/5
N2 - In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.
AB - In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.
KW - EPR (ESR) spectroscopy
KW - Intermediate filaments
KW - Site-directed spin labeling
KW - Vimentin
UR - http://www.scopus.com/inward/record.url?scp=34548324639&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548324639&partnerID=8YFLogxK
U2 - 10.1007/s12013-007-0035-4
DO - 10.1007/s12013-007-0035-4
M3 - Article
C2 - 17703067
AN - SCOPUS:34548324639
VL - 48
SP - 45
EP - 53
JO - Cell Biochemistry and Biophysics
JF - Cell Biochemistry and Biophysics
SN - 1085-9195
IS - 1
ER -