Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains

Madhu S. Budamagunta, John F. Hess, Paul G FitzGerald, John C Voss

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.

Original languageEnglish (US)
Pages (from-to)45-53
Number of pages9
JournalCell Biochemistry and Biophysics
Volume48
Issue number1
DOIs
StatePublished - May 2007

Keywords

  • EPR (ESR) spectroscopy
  • Intermediate filaments
  • Site-directed spin labeling
  • Vimentin

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Biophysics

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