Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains

Madhu S. Budamagunta, John F. Hess, Paul G FitzGerald, John C Voss

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.

Original languageEnglish (US)
Pages (from-to)45-53
Number of pages9
JournalCell Biochemistry and Biophysics
Volume48
Issue number1
DOIs
StatePublished - May 2007

Fingerprint

Intermediate Filament Proteins
Spin Labels
Intermediate Filaments
Electron Spin Resonance Spectroscopy
Vimentin
Paramagnetic resonance
Spectrum Analysis
Spectroscopy
Proteins
Agglomeration

Keywords

  • EPR (ESR) spectroscopy
  • Intermediate filaments
  • Site-directed spin labeling
  • Vimentin

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Biophysics

Cite this

Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains. / Budamagunta, Madhu S.; Hess, John F.; FitzGerald, Paul G; Voss, John C.

In: Cell Biochemistry and Biophysics, Vol. 48, No. 1, 05.2007, p. 45-53.

Research output: Contribution to journalArticle

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