Abstract
In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.
Original language | English (US) |
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Pages (from-to) | 45-53 |
Number of pages | 9 |
Journal | Cell Biochemistry and Biophysics |
Volume | 48 |
Issue number | 1 |
DOIs | |
State | Published - May 2007 |
Keywords
- EPR (ESR) spectroscopy
- Intermediate filaments
- Site-directed spin labeling
- Vimentin
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Biophysics