Abstract
In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 45-53 |
| Number of pages | 9 |
| Journal | Cell Biochemistry and Biophysics |
| Volume | 48 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 2007 |
Keywords
- EPR (ESR) spectroscopy
- Intermediate filaments
- Site-directed spin labeling
- Vimentin
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Biophysics
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Budamagunta, M. S., Hess, J. F., FitzGerald, P. G., & Voss, J. C. (2007). Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains. Cell Biochemistry and Biophysics, 48(1), 45-53. https://doi.org/10.1007/s12013-007-0035-4