Derivation and osmotolerance characterization of three immortalized tilapia (Oreochromis mossambicus) cell lines

Alison M. Gardell, Qin Qin, Robert H. Rice, Johnathan Li, Dietmar Kültz

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Fish cell cultures are becoming more widely used models for investigating molecular mechanisms of physiological response to environmental challenge. In this study, we derived two immortalized Mozambique tilapia ( Oreochromis mossambicus) cell lines from brain (OmB) and lip epithelium (OmL), and compared them to a previously immortalized bulbus arteriosus (TmB) cell line. The OmB and OmL cell lines were generated without or with Rho-associated kinase (ROCK) inhibitor/3T3 feeder layer supplementation. Although both approaches were successful, ROCK inhibitor/feeder layer supplementation was found to offer the advantages of selecting for epithelial-like cell type and decreasing time to immortalization. After immortalization (≥ passage 5), we characterized the proteomes of the newly derived cell lines (OmB and OmL) using LCMS and identified several unique cell markers for each line. Subsequently, osmotolerance for each of the three cell lines following acute exposure to elevated sodium chloride was evaluated. The acute maximum osmotolerance of these tilapia cell lines (>700 mOsm/kg) was markedly higher than that of any other known vertebrate cell line, but was significantly higher in the epithelial-like OmL cell line. To validate the physiological relevance of these tilapia cell lines, we quantified the effects of acute hyperosmotic challenge (450 mOsm/kg and 700 mOsm/kg) on the transcriptional regulation of two enzymes involved in biosynthesis of the compatible organic osmolyte, myo-inositol. Both enzymes were found to be robustly upregulated in all three tilapia cell lines. Therefore, the newly established tilapia cells lines represent valuable tools for studying molecular mechanisms involved in the osmotic stress response of euryhaline fish.

Original languageEnglish (US)
Article numbere95919
JournalPLoS One
Volume9
Issue number5
DOIs
StatePublished - May 5 2014

Fingerprint

Tilapia (Cichlidae)
Tilapia
osmotolerance
Oreochromis mossambicus
Cells
cell lines
Cell Line
lips
Lip
epithelium
Epithelium
Feeder Cells
rho-Associated Kinases
tilapia (common name)
Fish
Fishes
phosphotransferases (kinases)
euryhaline species
Osmoregulation
molecular models

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Derivation and osmotolerance characterization of three immortalized tilapia (Oreochromis mossambicus) cell lines. / Gardell, Alison M.; Qin, Qin; Rice, Robert H.; Li, Johnathan; Kültz, Dietmar.

In: PLoS One, Vol. 9, No. 5, e95919, 05.05.2014.

Research output: Contribution to journalArticle

Gardell, Alison M. ; Qin, Qin ; Rice, Robert H. ; Li, Johnathan ; Kültz, Dietmar. / Derivation and osmotolerance characterization of three immortalized tilapia (Oreochromis mossambicus) cell lines. In: PLoS One. 2014 ; Vol. 9, No. 5.
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