Depression of developing neuromuscular synapses induced by repetitive postsynaptic depolarizations

Yi Jiuan Lo, Yichun Lin, Dan H. Sanes, Mu Ming Poo

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Effect of postsynaptic activity on the synaptic efficacy was studied in Xenopus nerve-muscle cultures. Repetitive post-synaptic depolarizations induced by injection of current pulses into singly innervated myocytes resulted in significant reduction in the frequency of spontaneous synaptic currents and the amplitude of nerve-evoked synaptic currents at the majority of synapses that showed immature synaptic properties. Repetitive hyperpolarizations and steady depolarizations of similar duration were without effect. The depolarization-induced synaptic depression appeared to result predominately from a reduced ACh secretion from the presynaptic nerve terminal. Buffering the myocyte cytosolic Ca2+ at a low level with intracellular loading of a Ca2+ buffer, 1,2-bis(2-aminophenoxy)ethane- N,N,N,N-tetra-acetic acid (BAPTA), significantly reduced the effect of the depolarizations. Thus postsynaptic electrical activity can regulate the synaptic efficacy of the developing neuromuscular synapses and the regulation may be mediated by retrograde transsynaptic interactions.

Original languageEnglish (US)
Pages (from-to)4694-4704
Number of pages11
JournalJournal of Neuroscience
Volume14
Issue number8
StatePublished - Aug 1 1994
Externally publishedYes

Keywords

  • cell culture
  • neuromuscular junction
  • synaptic competition
  • synaptic plasticity
  • synaptogenesis
  • Xenopus laevis

ASJC Scopus subject areas

  • Neuroscience(all)

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