Colonies of human lymphocytes with thymus-derived (T) cell characteristics can be induced by phytohemagglutinin P to grow in a semisolid medium. To expand the data base on the effects of antimicrobial agents on cell-mediated immunity, the effect of 30 antimicrobial agents on T-lymphocyte cloning was studied. All of the drugs were added to the cultures in concentrations ranging from 10-5 to 10-14 M, and the results were compared with those in cultures without the drug. Drugs that inhibit protein synthesis at the 50S ribosomal subunit in bacteria - in particular, chloramphenicol, clindamycin, erythromycin, and troleandomycin - suppressed colony formation. However, the most significantly immunosuppressive agent was rifampin; it suppressed colony formation at concentrations of up to 2.5 x 10-9 M, a value significantly lower than that found in previous in vitro testing and well below therapeutic levels. Screening of drugs by lymphocyte cloning techniques for possible suppression of cell-mediated immunity appears to be a rapid, sensitive, and inexpensive procedure.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Infectious Diseases|
|State||Published - 1981|
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health