Abstract
The metal ion content of eighteen different lectins was determined. The lectins were demetallized and the binding activity of native and demetallized forms were investigated using non-denaturing polyacrylamide affinity gel electrophoresis. The binding activities of all lectins were dependent on their metal ion content; when the cations were removed the lectins lost their carbohydrate binding activity. There was a marked difference in the strength with which lectins bind divalent cations.
Original language | English (US) |
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Pages (from-to) | 1069-1074 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 115 |
Issue number | 3 |
DOIs | |
State | Published - Sep 30 1983 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology