Dependence on cations for the binding activity of lectins as determined by affinity electrophoresis

Bo Lönnerdal, Carl A K Borrebaeck, Marilynn E. Etzler, Bo Ersson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The metal ion content of eighteen different lectins was determined. The lectins were demetallized and the binding activity of native and demetallized forms were investigated using non-denaturing polyacrylamide affinity gel electrophoresis. The binding activities of all lectins were dependent on their metal ion content; when the cations were removed the lectins lost their carbohydrate binding activity. There was a marked difference in the strength with which lectins bind divalent cations.

Original languageEnglish (US)
Pages (from-to)1069-1074
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume115
Issue number3
DOIs
StatePublished - Sep 30 1983

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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