Dependence on cations for the binding activity of lectins as determined by affinity electrophoresis

Bo Lönnerdal, Carl A K Borrebaeck, Marilynn E. Etzler, Bo Ersson

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The metal ion content of eighteen different lectins was determined. The lectins were demetallized and the binding activity of native and demetallized forms were investigated using non-denaturing polyacrylamide affinity gel electrophoresis. The binding activities of all lectins were dependent on their metal ion content; when the cations were removed the lectins lost their carbohydrate binding activity. There was a marked difference in the strength with which lectins bind divalent cations.

Original languageEnglish (US)
Pages (from-to)1069-1074
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Sep 30 1983


ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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