Deoxyribonucleic acid flow cytometry of benign prostatic disease

A. D. Deitch, M. A. Strand, Ralph W deVere White

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Deoxyribonucleic acid flow cytometry was performed on aspirated cells from 198 patients who had benign cytological or histological findings. Unsatisfactory acellular histograms were obtained from 10.6 per cent of the cases. Three-fourths of the satisfactory samples (more than 5,000 cells after subtracting debris) showed the expected single peak deoxyribonucleic acid diploid to near diploid histograms. Unexpectedly, the remaining samples were deoxyribonucleic acid aneuploid, most having 2 peridiploid peaks (deoxyribonucleic acid index 0.82 to 1.31). Usually, proliferation was low with less than 20 per cent hyperdiploid cells and with 2.5 ± 1.5 per cent G2 cells. In 10 per cent of the single peak histograms there was evidence of inflammation, identified as an increase in hyperdiploid cells without an increased percentage of G2 cells but with a tail of high channel values. The aforementioned histogram features were considered to be benign findings. Seven per cent of the samples had deoxyribonucleic acid histograms suggestive of prostate cancer. Of these samples 7 had diploid or peridiploid aneuploid histograms with high proliferation (more than 20 per cent hyperdiploid cells with 8.5 ± 3.8 per cent G2 cells), while 5 had histograms with deoxyribonucleic acid aneuploidy other than peridiploidy.

Original languageEnglish (US)
Pages (from-to)759-762
Number of pages4
JournalJournal of Urology
Volume142
Issue number3
StatePublished - 1989

ASJC Scopus subject areas

  • Urology

Fingerprint Dive into the research topics of 'Deoxyribonucleic acid flow cytometry of benign prostatic disease'. Together they form a unique fingerprint.

Cite this