Demonstration that C-reactive protein decreases eNOS expression and bioactivity in human aortic endothelial cells

Senthil Kumar Venugopal, Sridevi Devaraj, Ivan Yuhanna, Philip Shaul, Ishwarlal Jialal

Research output: Contribution to journalArticle

681 Citations (Scopus)

Abstract

Background - C-reactive protein (CRP), the prototypic marker of inflammation, has been shown to be an independent predictor of cardiovascular events. Endothelial nitric oxide synthase (eNOS) deficiency is a pivotal event in atherogenesis. Methods and Results - We tested the effect of CRP on eNOS expression and bioactivity in cultured human aortic endothelial cells (HAECs). CRP decreased eNOS mRNA, protein abundance, and enzyme activity in HAECs. Furthermore, eNOS bioactivity assayed by cyclic GMP levels was significantly reduced by CRP. Preincubation of cells with CRP also significantly increased the adhesion of monocytes to HAECs. Conclusion - CRP causes a direct reduction in eNOS expression and bioactivity in HAECs, further supporting its role in atherogenesis.

Original languageEnglish (US)
Pages (from-to)1439-1441
Number of pages3
JournalCirculation
Volume106
Issue number12
DOIs
StatePublished - Sep 17 2002

Fingerprint

Nitric Oxide Synthase Type III
C-Reactive Protein
Endothelial Cells
Atherosclerosis
Cyclic GMP
Human Activities
Monocytes
Inflammation
Messenger RNA
Enzymes
Proteins

Keywords

  • C-reactive protein
  • Endothelium
  • Inflammation
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Demonstration that C-reactive protein decreases eNOS expression and bioactivity in human aortic endothelial cells. / Venugopal, Senthil Kumar; Devaraj, Sridevi; Yuhanna, Ivan; Shaul, Philip; Jialal, Ishwarlal.

In: Circulation, Vol. 106, No. 12, 17.09.2002, p. 1439-1441.

Research output: Contribution to journalArticle

Venugopal, Senthil Kumar ; Devaraj, Sridevi ; Yuhanna, Ivan ; Shaul, Philip ; Jialal, Ishwarlal. / Demonstration that C-reactive protein decreases eNOS expression and bioactivity in human aortic endothelial cells. In: Circulation. 2002 ; Vol. 106, No. 12. pp. 1439-1441.
@article{488b26fff9144dc3820a6414bb7ee277,
title = "Demonstration that C-reactive protein decreases eNOS expression and bioactivity in human aortic endothelial cells",
abstract = "Background - C-reactive protein (CRP), the prototypic marker of inflammation, has been shown to be an independent predictor of cardiovascular events. Endothelial nitric oxide synthase (eNOS) deficiency is a pivotal event in atherogenesis. Methods and Results - We tested the effect of CRP on eNOS expression and bioactivity in cultured human aortic endothelial cells (HAECs). CRP decreased eNOS mRNA, protein abundance, and enzyme activity in HAECs. Furthermore, eNOS bioactivity assayed by cyclic GMP levels was significantly reduced by CRP. Preincubation of cells with CRP also significantly increased the adhesion of monocytes to HAECs. Conclusion - CRP causes a direct reduction in eNOS expression and bioactivity in HAECs, further supporting its role in atherogenesis.",
keywords = "C-reactive protein, Endothelium, Inflammation, Nitric oxide synthase",
author = "Venugopal, {Senthil Kumar} and Sridevi Devaraj and Ivan Yuhanna and Philip Shaul and Ishwarlal Jialal",
year = "2002",
month = "9",
day = "17",
doi = "10.1161/01.CIR.0000033116.22237.F9",
language = "English (US)",
volume = "106",
pages = "1439--1441",
journal = "Circulation",
issn = "0009-7322",
publisher = "Lippincott Williams and Wilkins",
number = "12",

}

TY - JOUR

T1 - Demonstration that C-reactive protein decreases eNOS expression and bioactivity in human aortic endothelial cells

AU - Venugopal, Senthil Kumar

AU - Devaraj, Sridevi

AU - Yuhanna, Ivan

AU - Shaul, Philip

AU - Jialal, Ishwarlal

PY - 2002/9/17

Y1 - 2002/9/17

N2 - Background - C-reactive protein (CRP), the prototypic marker of inflammation, has been shown to be an independent predictor of cardiovascular events. Endothelial nitric oxide synthase (eNOS) deficiency is a pivotal event in atherogenesis. Methods and Results - We tested the effect of CRP on eNOS expression and bioactivity in cultured human aortic endothelial cells (HAECs). CRP decreased eNOS mRNA, protein abundance, and enzyme activity in HAECs. Furthermore, eNOS bioactivity assayed by cyclic GMP levels was significantly reduced by CRP. Preincubation of cells with CRP also significantly increased the adhesion of monocytes to HAECs. Conclusion - CRP causes a direct reduction in eNOS expression and bioactivity in HAECs, further supporting its role in atherogenesis.

AB - Background - C-reactive protein (CRP), the prototypic marker of inflammation, has been shown to be an independent predictor of cardiovascular events. Endothelial nitric oxide synthase (eNOS) deficiency is a pivotal event in atherogenesis. Methods and Results - We tested the effect of CRP on eNOS expression and bioactivity in cultured human aortic endothelial cells (HAECs). CRP decreased eNOS mRNA, protein abundance, and enzyme activity in HAECs. Furthermore, eNOS bioactivity assayed by cyclic GMP levels was significantly reduced by CRP. Preincubation of cells with CRP also significantly increased the adhesion of monocytes to HAECs. Conclusion - CRP causes a direct reduction in eNOS expression and bioactivity in HAECs, further supporting its role in atherogenesis.

KW - C-reactive protein

KW - Endothelium

KW - Inflammation

KW - Nitric oxide synthase

UR - http://www.scopus.com/inward/record.url?scp=0037126012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037126012&partnerID=8YFLogxK

U2 - 10.1161/01.CIR.0000033116.22237.F9

DO - 10.1161/01.CIR.0000033116.22237.F9

M3 - Article

VL - 106

SP - 1439

EP - 1441

JO - Circulation

JF - Circulation

SN - 0009-7322

IS - 12

ER -