Demonstration of coccidioides immitis and coccidioides posadasii DNA in soil samples collected from dinosaur national monument, Utah

Suzanne M. Johnson, Erin L. Carlson, Frederick S. Fisher, Demosthenes Pappagianis

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Soil samples were collected in 2006 from Dinosaur National Monument (DNM), Utah, the site of an outbreak of coccidioidomycosis in 2001. DNA was isolated from two soil samples, and polymerase chain reaction (PCR) amplified Coccidioides DNA present in both samples. Ribosomal RNA genes and internal transcribed spacer (ITS) region PCR products were sequenced. Single-nucleotide polymorphisms indicated that the DNA from sample SS06RH was that of Coccidioides immitis, while the DNA from sample SS06UM was C. posadasii. This is the first report to directly demonstrate Coccidioides in soils from DNM and the first to report the presence of both C. immitis and C. posadasii in the same geographic location.

Original languageEnglish (US)
Pages (from-to)610-617
Number of pages8
JournalMedical Mycology
Volume52
Issue number6
DOIs
StatePublished - 2014

Keywords

  • Coccidioides immitis
  • Coccidioides posadasii
  • DNA
  • ITS
  • PCR
  • polymorphism

ASJC Scopus subject areas

  • veterinary(all)
  • Infectious Diseases
  • Medicine(all)

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