Delivery of a live calf after in vitro maturation and fertilization of oocytes obtained from ovaries removed from a moribund cow.

E. Behboodi, D. C. Van Metre, Robert Bondurant, G. B. Anderson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An aged Holstein cow of high genetic merit and economic value was determined to have a squamous cell carcinoma of the tarsus. Treatment was unsuccessful, as was an attempt at superovulation and embryo collection; thus, the owner elected euthanasia. On day 17 of the estrous cycle, the ovaries were removed via laparotomy and the cow was immediately euthanatized. Oocytes were aspirated from ovarian follicles and subjected to in vitro maturation and fertilization procedures. Seven morulae that developed in culture were transferred nonsurgically to 3 recipient females, resulting in birth of a full-term healthy male calf. In vitro maturation and fertilization procedures can be used to obtain offspring from valuable, terminally ill cows for which conventional embryo collection techniques have proven unsuccessful.

Original languageEnglish (US)
Pages (from-to)1588-1589
Number of pages2
JournalJournal of the American Veterinary Medical Association
Volume205
Issue number11
StatePublished - Dec 1 1994

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In Vitro Oocyte Maturation Techniques
Fertilization in Vitro
fertilization (reproduction)
Ovary
oocytes
Embryonic Structures
calves
Superovulation
Morula
cows
Terminally Ill
Ovarian Follicle
Euthanasia
Estrous Cycle
embryo (animal)
Ankle
Laparotomy
Oocytes
Squamous Cell Carcinoma
genetic merit

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Delivery of a live calf after in vitro maturation and fertilization of oocytes obtained from ovaries removed from a moribund cow. / Behboodi, E.; Van Metre, D. C.; Bondurant, Robert; Anderson, G. B.

In: Journal of the American Veterinary Medical Association, Vol. 205, No. 11, 01.12.1994, p. 1588-1589.

Research output: Contribution to journalArticle

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