Delineation of joint molecule resolution pathways in meiosis identifies a crossover-specific resolvase

Kseniya Zakharyevich, Shangming Tang, Yunmei Ma, Neil Hunter

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

At the final step of homologous recombination, Holliday junction-containing joint molecules (JMs) are resolved to form crossover or noncrossover products. The enzymes responsible for JM resolution in vivo remain uncertain, but three distinct endonucleases capable of resolving JMs in vitro have been identified: Mus81-Mms4(EME1), Slx1-Slx4(BTBD12), and Yen1(GEN1). Using physical monitoring of recombination during budding yeast meiosis, we show that all three endonucleases are capable of promoting JM resolution in vivo. However, in mms4 slx4 yen1 triple mutants, JM resolution and crossing over occur efficiently. Paradoxically, crossing over in this background is strongly dependent on the Blooms helicase ortholog Sgs1, a component of a well-characterized anticrossover activity. Sgs1-dependent crossing over, but not JM resolution per se, also requires XPG family nuclease Exo1 and the MutLγ complex Mlh1-Mlh3. Thus, Sgs1, Exo1, and MutLγ together define a previously undescribed meiotic JM resolution pathway that produces the majority of crossovers in budding yeast and, by inference, in mammals.

Original languageEnglish (US)
Pages (from-to)334-347
Number of pages14
JournalCell
Volume149
Issue number2
DOIs
StatePublished - Apr 13 2012

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Recombinases
Meiosis
Joints
Molecules
Saccharomycetales
Endonucleases
Yeast
Cruciform DNA
Mammals
Homologous Recombination
Genetic Recombination
Monitoring
Enzymes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Delineation of joint molecule resolution pathways in meiosis identifies a crossover-specific resolvase. / Zakharyevich, Kseniya; Tang, Shangming; Ma, Yunmei; Hunter, Neil.

In: Cell, Vol. 149, No. 2, 13.04.2012, p. 334-347.

Research output: Contribution to journalArticle

Zakharyevich, Kseniya ; Tang, Shangming ; Ma, Yunmei ; Hunter, Neil. / Delineation of joint molecule resolution pathways in meiosis identifies a crossover-specific resolvase. In: Cell. 2012 ; Vol. 149, No. 2. pp. 334-347.
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