Delineation of antigen-specific and antigen-nonspecific CD8+ memory T-cell responses after cytokine-based cancer immunotherapy

Julia K. Tietze, Danice E C Wilkins, Gail D. Sckisel, Myriam N. Bouchlaka, Kory L. Alderson, Jonathan M. Weiss, Erik Ames, Kevin W. Bruhn, Noah Craft, Robert H. Wiltrout, Dan L. Longo, Lewis L. Lanier, Bruce R. Blazar, Doug Redelman, William J Murphy

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Memory T cells exhibit tremendous antigen specificity within the immune system and accumulate with age. Our studies reveal an antigen-independent expansion of memory, but not naive, CD8+ T cells after several immunotherapeutic regimens for cancer resulting in a distinctive phenotype. Signaling through T-cell receptors (TCRs) or CD3 in both mouse and human memory CD8+ T cells markedly up-regulated programmed death-1 (PD-1) and CD25 (IL-2 receptor α chain), and led to antigen-specific tumor cell killing. In contrast, exposure to cytokine alone in vitro or with immunotherapy in vivo did not up-regulate these markers but resulted in expanded memory CD8 + T cells expressing NKG2D, granzyme B, and possessing broadly lytic capabilities. Blockade of NKG2D in mice also resulted in significantly diminished antitumor effects after immunotherapy. Treatment of TCR-transgenic mice bearing nonantigen expressing tumors with immunotherapy still resulted in significant antitumor effects. Human melanoma tissue biopsies obtained from patients after topically applied immunodulatory treatment resulted in increased numbers of these CD8+ CD25- cells within the tumor site. These findings demonstrate that memory CD8+ T cells can express differential phenotypes indicative of adaptive or innate effectors based on the nature of the stimuli in a process conserved across species.

Original languageEnglish (US)
Pages (from-to)3073-3083
Number of pages11
JournalBlood
Volume119
Issue number13
DOIs
StatePublished - Mar 29 2012

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

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    Tietze, J. K., Wilkins, D. E. C., Sckisel, G. D., Bouchlaka, M. N., Alderson, K. L., Weiss, J. M., Ames, E., Bruhn, K. W., Craft, N., Wiltrout, R. H., Longo, D. L., Lanier, L. L., Blazar, B. R., Redelman, D., & Murphy, W. J. (2012). Delineation of antigen-specific and antigen-nonspecific CD8+ memory T-cell responses after cytokine-based cancer immunotherapy. Blood, 119(13), 3073-3083. https://doi.org/10.1182/blood-2011-07-369736