Abstract
Nonsteroidal anti-inflammatory drugs ameliorate pain and fever by inhibiting cyclooxygenase (COX) and suppressing prostanoid formation. Microsomal prostaglandin E synthase-1 (mPGES-1) catalyzes formation of PGE2 from the COX product PGH2 and has emerged as a therapeutic target. Inhibition of mPGES-1, however, renders the PGH2 substrate available for diversion to other PG synthases. To address the possibility that substrate diversion augments formation of PGs that might modulate bronchial tone, we assessed the impact of mPGES-1 deletion in a mouse model of ozone-induced airway hyper-responsiveness. Ozone exposure increased total lung resistance to inhaled methacholine in wild-type mice. Deletion of mPGES-1 had little effect on total lung resistance in either naive or ozone-exposed animals. The carbachol-induced narrowing of luminal diameter in intrapulmonary airways of lung slices from acute ozone-exposed mice was also unaltered by mPGES-1 deletion. Likewise, although concentrations of PGE2 were reduced in bronchoalveolar lavage fluid, whereas 6-keto-PGF1α, PGD2, and PGF2α, all were increased, deletion of mPGES-1 failed to influence cell trafficking into the airways of either naive or ozone-exposed animals. Despite biochemical evidence of PGH2 substrate diversion to potential bronchomodulator PGs, deletion of mPGES-1 had little effect on ozone-induced airway inflammation or airway hyper-responsiveness. Pharmacologically targeting mPGES-1 may not predispose patients at risk to airway dysfunction.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 63-68 |
| Number of pages | 6 |
| Journal | Journal of Pharmacology and Experimental Therapeutics |
| Volume | 334 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pharmacology
- Molecular Medicine
Cite this
Deletion of microsomal prostaglandin E synthase-1 does not alter ozone-induced airway hyper-responsiveness. / Wang, Miao; Cooper, Philip R.; Jiang, Meiqi; Zhao, Hengjiang; Hui, Yiqun; Yao, Yubing; Tate, Joshua C.; Damera, Gautam; Lawson, John A.; Jester, William F.; Haczku, Angela Franciska; Panettieri, Reynold A.; FitzGerald, Garret A.
In: Journal of Pharmacology and Experimental Therapeutics, Vol. 334, No. 1, 07.2010, p. 63-68.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Deletion of microsomal prostaglandin E synthase-1 does not alter ozone-induced airway hyper-responsiveness
AU - Wang, Miao
AU - Cooper, Philip R.
AU - Jiang, Meiqi
AU - Zhao, Hengjiang
AU - Hui, Yiqun
AU - Yao, Yubing
AU - Tate, Joshua C.
AU - Damera, Gautam
AU - Lawson, John A.
AU - Jester, William F.
AU - Haczku, Angela Franciska
AU - Panettieri, Reynold A.
AU - FitzGerald, Garret A.
PY - 2010/7
Y1 - 2010/7
N2 - Nonsteroidal anti-inflammatory drugs ameliorate pain and fever by inhibiting cyclooxygenase (COX) and suppressing prostanoid formation. Microsomal prostaglandin E synthase-1 (mPGES-1) catalyzes formation of PGE2 from the COX product PGH2 and has emerged as a therapeutic target. Inhibition of mPGES-1, however, renders the PGH2 substrate available for diversion to other PG synthases. To address the possibility that substrate diversion augments formation of PGs that might modulate bronchial tone, we assessed the impact of mPGES-1 deletion in a mouse model of ozone-induced airway hyper-responsiveness. Ozone exposure increased total lung resistance to inhaled methacholine in wild-type mice. Deletion of mPGES-1 had little effect on total lung resistance in either naive or ozone-exposed animals. The carbachol-induced narrowing of luminal diameter in intrapulmonary airways of lung slices from acute ozone-exposed mice was also unaltered by mPGES-1 deletion. Likewise, although concentrations of PGE2 were reduced in bronchoalveolar lavage fluid, whereas 6-keto-PGF1α, PGD2, and PGF2α, all were increased, deletion of mPGES-1 failed to influence cell trafficking into the airways of either naive or ozone-exposed animals. Despite biochemical evidence of PGH2 substrate diversion to potential bronchomodulator PGs, deletion of mPGES-1 had little effect on ozone-induced airway inflammation or airway hyper-responsiveness. Pharmacologically targeting mPGES-1 may not predispose patients at risk to airway dysfunction.
AB - Nonsteroidal anti-inflammatory drugs ameliorate pain and fever by inhibiting cyclooxygenase (COX) and suppressing prostanoid formation. Microsomal prostaglandin E synthase-1 (mPGES-1) catalyzes formation of PGE2 from the COX product PGH2 and has emerged as a therapeutic target. Inhibition of mPGES-1, however, renders the PGH2 substrate available for diversion to other PG synthases. To address the possibility that substrate diversion augments formation of PGs that might modulate bronchial tone, we assessed the impact of mPGES-1 deletion in a mouse model of ozone-induced airway hyper-responsiveness. Ozone exposure increased total lung resistance to inhaled methacholine in wild-type mice. Deletion of mPGES-1 had little effect on total lung resistance in either naive or ozone-exposed animals. The carbachol-induced narrowing of luminal diameter in intrapulmonary airways of lung slices from acute ozone-exposed mice was also unaltered by mPGES-1 deletion. Likewise, although concentrations of PGE2 were reduced in bronchoalveolar lavage fluid, whereas 6-keto-PGF1α, PGD2, and PGF2α, all were increased, deletion of mPGES-1 failed to influence cell trafficking into the airways of either naive or ozone-exposed animals. Despite biochemical evidence of PGH2 substrate diversion to potential bronchomodulator PGs, deletion of mPGES-1 had little effect on ozone-induced airway inflammation or airway hyper-responsiveness. Pharmacologically targeting mPGES-1 may not predispose patients at risk to airway dysfunction.
UR - http://www.scopus.com/inward/record.url?scp=77953770999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953770999&partnerID=8YFLogxK
U2 - 10.1124/jpet.110.166678
DO - 10.1124/jpet.110.166678
M3 - Article
C2 - 20371706
AN - SCOPUS:77953770999
VL - 334
SP - 63
EP - 68
JO - Journal of Pharmacology and Experimental Therapeutics
JF - Journal of Pharmacology and Experimental Therapeutics
SN - 0022-3565
IS - 1
ER -