Deletion of leptin receptors in vagal afferent neurons disrupts estrogen signaling, body weight, food intake and hormonal controls of feeding in female mice

Kuei Pin Huang, Charlotte C. Ronveaux, Guillaume de Lartigue, Nori Geary, Lori Asarian, Helen E Raybould

Research output: Contribution to journalArticle

Abstract

Deletion of the leptin receptor from vagal afferent neurons (VAN) using a conditional deletion (Nav1.8/LepRfl/fl) results in an obese phenotype with increased food intake and lack of exogenous cholecystokinin (CCK)-induced satiation in male mice. Female mice are partially protected from weight gain and increased food intake in response to ingestion of high-fat (HF) diets. However, whether the lack of leptin signaling in VAN leads to an obese phenotype or disruption of hypothalamic-pituitary-gonadal axis function in female mice is unclear. Here, we tested the hypothesis that leptin signaling in VAN is essential to maintain estrogen signaling and control of food intake, energy expenditure, and adiposity in female mice. Female Nav1.8/LepRfl/fl mice gained more weight, had increased gonadal fat mass, increased meal number in the dark phase, and increased total food intake compared with wild-type controls. Resting energy expenditure was unaffected. The decrease in food intake produced by intraperitoneal injection of CCK (3 μg/kg body wt) was attenuated in female Nav1.8/LepRfl/fl mice compared with wild-type controls. Intraperitoneal injection of ghrelin (100 μg/kg body wt) increased food intake in Nav1.8/LepRfl/fl mice but not in wild-type controls. Ovarian steroidogenesis was suppressed, resulting in decreased plasma estradiol, which was accompanied by decreased expression of estrogen receptor-1 (Esr1) in VAN but not in the hypothalamic arcuate nucleus. These data suggest that the absence of leptin signaling in VAN is accompanied by disruption of estrogen signaling in female mice, leading to an obese phenotype possibly via altered control of feeding behavior.

Original languageEnglish (US)
Pages (from-to)E568-E577
JournalAmerican journal of physiology. Endocrinology and metabolism
Volume316
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Leptin Receptors
Afferent Neurons
Estrogens
Eating
Body Weight
Leptin
Cholecystokinin
Intraperitoneal Injections
Phenotype
Energy Metabolism
Satiation
Arcuate Nucleus of Hypothalamus
Ghrelin
Estrogen Receptor alpha
Adiposity
High Fat Diet
Feeding Behavior
Weight Gain
Meals
Estradiol

Keywords

  • cholecystokinin
  • estradiol
  • estrogen receptor 1
  • leptin receptor
  • vagal afferent

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Deletion of leptin receptors in vagal afferent neurons disrupts estrogen signaling, body weight, food intake and hormonal controls of feeding in female mice. / Huang, Kuei Pin; Ronveaux, Charlotte C.; de Lartigue, Guillaume; Geary, Nori; Asarian, Lori; Raybould, Helen E.

In: American journal of physiology. Endocrinology and metabolism, Vol. 316, No. 4, 01.04.2019, p. E568-E577.

Research output: Contribution to journalArticle

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