Delayed seroconversion following naturally acquired caprine arthritis-encephalitis virus infection in goats.

E. Rimstad, Nancy East, M. Torten, J. Higgins, E. DeRock, Niels C Pedersen

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

One hundred eight milking goats from a dairy that had been using a modified caprine arthritis-encephalitis virus (CAEV) eradication program were tested for CAEV antibodies by serologic methods and for proviral CAEV DNA by use of polymerase chain reaction (PCR) technology. All goats were free of clinical symptoms of CAEV infection. Twenty-seven of the 108 goats were considered seropositive, on the basis of ELISA results. Proviral CAEV DNA was detected, using PCR techniques, in mononuclear leukocytes in blood samples obtained from 25 of the these 27 seropositive goats. Twenty of the 81 seronegative goats also had positive PCR test results. Ten of these goats seroconverted by 8 months later, and virus was readily isolated from mononuclear leukocytes in venous blood samples after the goats had seroconverted. Virus was also isolated from mononuclear leukocytes in blood samples collected from 4 of 11 goats that were seronegative, but had positive PCR test results. These results indicated that seroconversion can be delayed for many months following natural infection with CAEV. Delayed seroconversion appears to be a feature of CAEV infection, which may have direct implications for CAEV eradication programs and epidemiologic studies that rely on serologic methods to detect infected goats.

Original languageEnglish (US)
Pages (from-to)1858-1862
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume54
Issue number11
StatePublished - Nov 1993

Fingerprint

Caprine Arthritis-Encephalitis Viruses
Caprine arthritis encephalitis virus
seroconversion
Virus Diseases
Goats
goats
infection
Mononuclear Leukocytes
mononuclear leukocytes
polymerase chain reaction
Polymerase Chain Reaction
blood
Viruses
Seroconversion
Infectious Encephalitis
viruses
DNA
DNA-Directed DNA Polymerase
milking
sampling

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Delayed seroconversion following naturally acquired caprine arthritis-encephalitis virus infection in goats. / Rimstad, E.; East, Nancy; Torten, M.; Higgins, J.; DeRock, E.; Pedersen, Niels C.

In: American Journal of Veterinary Research, Vol. 54, No. 11, 11.1993, p. 1858-1862.

Research output: Contribution to journalArticle

@article{a1b30d9485234aa8bcab936bc6b30119,
title = "Delayed seroconversion following naturally acquired caprine arthritis-encephalitis virus infection in goats.",
abstract = "One hundred eight milking goats from a dairy that had been using a modified caprine arthritis-encephalitis virus (CAEV) eradication program were tested for CAEV antibodies by serologic methods and for proviral CAEV DNA by use of polymerase chain reaction (PCR) technology. All goats were free of clinical symptoms of CAEV infection. Twenty-seven of the 108 goats were considered seropositive, on the basis of ELISA results. Proviral CAEV DNA was detected, using PCR techniques, in mononuclear leukocytes in blood samples obtained from 25 of the these 27 seropositive goats. Twenty of the 81 seronegative goats also had positive PCR test results. Ten of these goats seroconverted by 8 months later, and virus was readily isolated from mononuclear leukocytes in venous blood samples after the goats had seroconverted. Virus was also isolated from mononuclear leukocytes in blood samples collected from 4 of 11 goats that were seronegative, but had positive PCR test results. These results indicated that seroconversion can be delayed for many months following natural infection with CAEV. Delayed seroconversion appears to be a feature of CAEV infection, which may have direct implications for CAEV eradication programs and epidemiologic studies that rely on serologic methods to detect infected goats.",
author = "E. Rimstad and Nancy East and M. Torten and J. Higgins and E. DeRock and Pedersen, {Niels C}",
year = "1993",
month = "11",
language = "English (US)",
volume = "54",
pages = "1858--1862",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "11",

}

TY - JOUR

T1 - Delayed seroconversion following naturally acquired caprine arthritis-encephalitis virus infection in goats.

AU - Rimstad, E.

AU - East, Nancy

AU - Torten, M.

AU - Higgins, J.

AU - DeRock, E.

AU - Pedersen, Niels C

PY - 1993/11

Y1 - 1993/11

N2 - One hundred eight milking goats from a dairy that had been using a modified caprine arthritis-encephalitis virus (CAEV) eradication program were tested for CAEV antibodies by serologic methods and for proviral CAEV DNA by use of polymerase chain reaction (PCR) technology. All goats were free of clinical symptoms of CAEV infection. Twenty-seven of the 108 goats were considered seropositive, on the basis of ELISA results. Proviral CAEV DNA was detected, using PCR techniques, in mononuclear leukocytes in blood samples obtained from 25 of the these 27 seropositive goats. Twenty of the 81 seronegative goats also had positive PCR test results. Ten of these goats seroconverted by 8 months later, and virus was readily isolated from mononuclear leukocytes in venous blood samples after the goats had seroconverted. Virus was also isolated from mononuclear leukocytes in blood samples collected from 4 of 11 goats that were seronegative, but had positive PCR test results. These results indicated that seroconversion can be delayed for many months following natural infection with CAEV. Delayed seroconversion appears to be a feature of CAEV infection, which may have direct implications for CAEV eradication programs and epidemiologic studies that rely on serologic methods to detect infected goats.

AB - One hundred eight milking goats from a dairy that had been using a modified caprine arthritis-encephalitis virus (CAEV) eradication program were tested for CAEV antibodies by serologic methods and for proviral CAEV DNA by use of polymerase chain reaction (PCR) technology. All goats were free of clinical symptoms of CAEV infection. Twenty-seven of the 108 goats were considered seropositive, on the basis of ELISA results. Proviral CAEV DNA was detected, using PCR techniques, in mononuclear leukocytes in blood samples obtained from 25 of the these 27 seropositive goats. Twenty of the 81 seronegative goats also had positive PCR test results. Ten of these goats seroconverted by 8 months later, and virus was readily isolated from mononuclear leukocytes in venous blood samples after the goats had seroconverted. Virus was also isolated from mononuclear leukocytes in blood samples collected from 4 of 11 goats that were seronegative, but had positive PCR test results. These results indicated that seroconversion can be delayed for many months following natural infection with CAEV. Delayed seroconversion appears to be a feature of CAEV infection, which may have direct implications for CAEV eradication programs and epidemiologic studies that rely on serologic methods to detect infected goats.

UR - http://www.scopus.com/inward/record.url?scp=0027689996&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027689996&partnerID=8YFLogxK

M3 - Article

VL - 54

SP - 1858

EP - 1862

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 11

ER -