Delayed cell cycle progression from SEPW1 depletion is p53- and p21-dependent in MCF-7 breast cancer cells

Wayne Chris Hawkes, Zeynep Alkan

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Selenium (Se) is an essential redox-active trace element with close connections to cancer. Most of Se's biological functions have been attributed to the antioxidant properties of Se-containing proteins. However, the relative contribution of selenoproteins and small Se compounds in cancer protection is still a matter of debate. The tumor suppressor p53 is the most frequently mutated gene in human cancer and is often referred to as the " guardian of the genome" In response to genomic stresses, p53 causes cell cycle arrest to allow time for genomic damage to be repaired before cell division or induces apoptosis to eliminate irreparably damaged cells. Selenoprotein W (SEPW1) is a highly conserved small thioredoxin-like protein required for cell cycle progression. The present work shows that SEPW1 facilitates the G1 to S-phase transition by down-regulating expression of the cyclin-dependent kinase inhibitor p21. SEPW1 controls p21 by modulating levels of the p53 transcription factor, and this is associated with changes in phosphorylation of Ser-33 in p53. More work is needed to identify the mechanism by which SEPW1 regulates phosphorylation of Ser-33 and the kinase or phosphatase enzymes involved.

Original languageEnglish (US)
Pages (from-to)36-40
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume413
Issue number1
DOIs
StatePublished - Sep 16 2011

Fingerprint

Cell Cycle
Phosphorylation
Cells
Breast Neoplasms
Selenium
Selenoprotein W
Genes
Selenium Compounds
Selenoproteins
Cyclin-Dependent Kinase Inhibitor p21
Neoplasms
Thioredoxins
Trace Elements
Phosphoric Monoester Hydrolases
Cell Cycle Proteins
Tumors
Phase Transition
Proteins
Transcription Factors
Phosphotransferases

Keywords

  • Cell cycle
  • P21 Cyclin-dependent kinase inhibitor
  • P53 Tumor suppressor protein
  • Selenium

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Delayed cell cycle progression from SEPW1 depletion is p53- and p21-dependent in MCF-7 breast cancer cells. / Hawkes, Wayne Chris; Alkan, Zeynep.

In: Biochemical and Biophysical Research Communications, Vol. 413, No. 1, 16.09.2011, p. 36-40.

Research output: Contribution to journalArticle

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