Defective removal of DNA cross-links in a repair-deficient mutant of Chinese hamster cells

R. E. Meyn, S. F. Jenkins, L. H. Thompson

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

To further understand the relationships between DNA damage, DNA repair, and cellular end points such as survival and mutation, the repair capacity of a DNA repair-deficient mutant (strain UV-20) of Chinese hamster ovary cells was characterized in response to DNA cross-linking agents. This mutant, previously shown to be hypersensitive to killing by both ultraviolet light and the cross-linking agent mitomycin C, was also found to be extremely sensitive to cis-diamminedichloroplatinum, another DNA cross-linking agent. The efficiency of DNA cross-link removal after treatment with mitomycin C or cis-diamminedichloroplatinum was measured using the technique of alkaline elution and compared in wild-type Chinese hamster ovary cells and strain UV-20. Wild-type cells removed 80 or 95% of the cross-links within 24 hr after treatment with cis-diamminedichloroplatinum or mitomycin C, respectively. In contrast, UV-20 cells, which were equally as susceptible to cross-link damage as were wild-type cells, removed only a small proportion of the cross-links made by either agent. These results emphasize the importance of DNA repair processes in modulating the cytotoxic effects of chemicals that produce DNA cross-link damage and suggest that cross-link repair in Chinese hamster ovary cells is controlled by a pathway that also repairs damage from ultraviolet radiation.

Original languageEnglish (US)
Pages (from-to)3106-3110
Number of pages5
JournalCancer Research
Volume42
Issue number8
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Meyn, R. E., Jenkins, S. F., & Thompson, L. H. (1982). Defective removal of DNA cross-links in a repair-deficient mutant of Chinese hamster cells. Cancer Research, 42(8), 3106-3110.