Abstract
Using in vitro lymphocyte proliferation induced by the phytomitogen concanavalin A (ConA), we investigated immune function and regulation in patients with myasthenia gravis (MG) and multiple sclerosis (MS). Unfractionated peripheral blood mononuclear cells of normal individuals responded to a wide range of ConA concentrations; the T cell fraction responded to a lesser degree and only to high concentrations. These findings suggest the presence of two receptors for ConA, one of high affinity present on a non-T cell accessory cell and the other of low affinity present on T cells. Contrasting defects in the level of response of unfractionated lymphocytes and T cells were found in patients with MG and MS. The peak response of T cells in the MG patients was 22.6 ± 9.6 x 103 cpm (mean ± SEM) compared with 54.6 ± 6.5 x 103 for controls (p < 0.05), while the response of unfractionated lymphocytes did not differ from that in controls. For MS patients, the unfractionated lymphocyte response was diminished; 56.3 ± 2.8 x 103 cpm versus 70.5 ± 4.5 x 103 for controls (p < 0.05), while the T cell response was normal. These results indicate a defect in the direct T cell response in MG; in contrast, in MS the response requiring T cell-accessory cell interaction is abnormal.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 456-462 |
| Number of pages | 7 |
| Journal | Annals of Neurology |
| Volume | 11 |
| Issue number | 5 |
| State | Published - 1982 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
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Defective mitogenic responses in myasthenia gravis and multiple sclerosis. / Dropcho, E. J.; Richman, David P; Antel, J. P.; Arnason, B. G W.
In: Annals of Neurology, Vol. 11, No. 5, 1982, p. 456-462.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Defective mitogenic responses in myasthenia gravis and multiple sclerosis
AU - Dropcho, E. J.
AU - Richman, David P
AU - Antel, J. P.
AU - Arnason, B. G W
PY - 1982
Y1 - 1982
N2 - Using in vitro lymphocyte proliferation induced by the phytomitogen concanavalin A (ConA), we investigated immune function and regulation in patients with myasthenia gravis (MG) and multiple sclerosis (MS). Unfractionated peripheral blood mononuclear cells of normal individuals responded to a wide range of ConA concentrations; the T cell fraction responded to a lesser degree and only to high concentrations. These findings suggest the presence of two receptors for ConA, one of high affinity present on a non-T cell accessory cell and the other of low affinity present on T cells. Contrasting defects in the level of response of unfractionated lymphocytes and T cells were found in patients with MG and MS. The peak response of T cells in the MG patients was 22.6 ± 9.6 x 103 cpm (mean ± SEM) compared with 54.6 ± 6.5 x 103 for controls (p < 0.05), while the response of unfractionated lymphocytes did not differ from that in controls. For MS patients, the unfractionated lymphocyte response was diminished; 56.3 ± 2.8 x 103 cpm versus 70.5 ± 4.5 x 103 for controls (p < 0.05), while the T cell response was normal. These results indicate a defect in the direct T cell response in MG; in contrast, in MS the response requiring T cell-accessory cell interaction is abnormal.
AB - Using in vitro lymphocyte proliferation induced by the phytomitogen concanavalin A (ConA), we investigated immune function and regulation in patients with myasthenia gravis (MG) and multiple sclerosis (MS). Unfractionated peripheral blood mononuclear cells of normal individuals responded to a wide range of ConA concentrations; the T cell fraction responded to a lesser degree and only to high concentrations. These findings suggest the presence of two receptors for ConA, one of high affinity present on a non-T cell accessory cell and the other of low affinity present on T cells. Contrasting defects in the level of response of unfractionated lymphocytes and T cells were found in patients with MG and MS. The peak response of T cells in the MG patients was 22.6 ± 9.6 x 103 cpm (mean ± SEM) compared with 54.6 ± 6.5 x 103 for controls (p < 0.05), while the response of unfractionated lymphocytes did not differ from that in controls. For MS patients, the unfractionated lymphocyte response was diminished; 56.3 ± 2.8 x 103 cpm versus 70.5 ± 4.5 x 103 for controls (p < 0.05), while the T cell response was normal. These results indicate a defect in the direct T cell response in MG; in contrast, in MS the response requiring T cell-accessory cell interaction is abnormal.
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M3 - Article
C2 - 6980619
AN - SCOPUS:0020078767
VL - 11
SP - 456
EP - 462
JO - Annals of Neurology
JF - Annals of Neurology
SN - 0364-5134
IS - 5
ER -