Abstract
We determined the gender of deer samples using polymerase chain reaction primers to the sry gene located on the Y chromosome of mammalian males. Primers to the ZFX/ZFY genes were added to the same amplification reaction as a control against amplification failure. The sry fragment amplified from deer DNA was partially sequenced. Sequence alignment with published bp sequences from other mammals and get migration indicate the fragment is 174 bp long. In a blind test, tissue samples from 100 individual deer and elk of known sex were correctly gender-typed. DNA from deer bloodstains and hair samples was successfully amplified and typed. Under known limiting DNA conditions, the duplex reaction results were reliable and no false positive females were observed. DNA detection on gels stained with SYBR green was more sensitive than with ethidium bromide. DNA from wildlife forensic samples confiscated between 1990 and 1997 was extracted with Chelex 100 and tested. Twenty-four out of 31 different evidence items tested were successfully gender-typed: 15 out of 21 bloodstains, 5 out of 6 hairs, and 4 out of 4 tissue samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 159-169 |
| Number of pages | 11 |
| Journal | California Fish and Game |
| Volume | 84 |
| Issue number | 4 |
| State | Published - Sep 1998 |
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ASJC Scopus subject areas
- Animal Science and Zoology
- Aquatic Science
Cite this
Deer gender determination by polymerase chain reaction : Validation study and application to tissues, bloodstains, and hair forensic samples from California. / Gilson, Arlette; Syvanen, Michael; Levine, Kenneth; Banks, James.
In: California Fish and Game, Vol. 84, No. 4, 09.1998, p. 159-169.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Deer gender determination by polymerase chain reaction
T2 - Validation study and application to tissues, bloodstains, and hair forensic samples from California
AU - Gilson, Arlette
AU - Syvanen, Michael
AU - Levine, Kenneth
AU - Banks, James
PY - 1998/9
Y1 - 1998/9
N2 - We determined the gender of deer samples using polymerase chain reaction primers to the sry gene located on the Y chromosome of mammalian males. Primers to the ZFX/ZFY genes were added to the same amplification reaction as a control against amplification failure. The sry fragment amplified from deer DNA was partially sequenced. Sequence alignment with published bp sequences from other mammals and get migration indicate the fragment is 174 bp long. In a blind test, tissue samples from 100 individual deer and elk of known sex were correctly gender-typed. DNA from deer bloodstains and hair samples was successfully amplified and typed. Under known limiting DNA conditions, the duplex reaction results were reliable and no false positive females were observed. DNA detection on gels stained with SYBR green was more sensitive than with ethidium bromide. DNA from wildlife forensic samples confiscated between 1990 and 1997 was extracted with Chelex 100 and tested. Twenty-four out of 31 different evidence items tested were successfully gender-typed: 15 out of 21 bloodstains, 5 out of 6 hairs, and 4 out of 4 tissue samples.
AB - We determined the gender of deer samples using polymerase chain reaction primers to the sry gene located on the Y chromosome of mammalian males. Primers to the ZFX/ZFY genes were added to the same amplification reaction as a control against amplification failure. The sry fragment amplified from deer DNA was partially sequenced. Sequence alignment with published bp sequences from other mammals and get migration indicate the fragment is 174 bp long. In a blind test, tissue samples from 100 individual deer and elk of known sex were correctly gender-typed. DNA from deer bloodstains and hair samples was successfully amplified and typed. Under known limiting DNA conditions, the duplex reaction results were reliable and no false positive females were observed. DNA detection on gels stained with SYBR green was more sensitive than with ethidium bromide. DNA from wildlife forensic samples confiscated between 1990 and 1997 was extracted with Chelex 100 and tested. Twenty-four out of 31 different evidence items tested were successfully gender-typed: 15 out of 21 bloodstains, 5 out of 6 hairs, and 4 out of 4 tissue samples.
UR - http://www.scopus.com/inward/record.url?scp=0032157950&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032157950&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0032157950
VL - 84
SP - 159
EP - 169
JO - California Fish and Game
JF - California Fish and Game
SN - 0008-1078
IS - 4
ER -