Decreased expression of the actin-binding protein gelsolin in endometrial and ovarian adenocarcinomas

Alaa M Afify, Bruce A. Werness

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Malignant cells are characterized by abnormalities of the cytoskeleton, and actin filaments in particular show marked disturbances of this normally well-organized network. Of the many actin-binding proteins, gelsolin plays a particularly important role in the remodeling of the actin cytoskeleton. Gelsolin expression is lost in transformed cells and in carcinomas of the breast and bladder, suggesting a role for gelsolin as a tumor suppressor. Because other human cancers have not yet been studied, we analyzed carcinomas of the endometrium and ovary for changes in gelsolin expression. Normal endometrial glands were positive in the secretory phase, whereas proliferative glands had lost expression. Although both simple and complex endometrial hyperplasias without atypia expressed gelsolin, some complex hyperplasias with atypia lost gelsolin expression. Expression in endometrial adenocarcinomas was related to grade; 11 of 14 grade II and III tumors were negative compared with 0 of 2 grade I tumors. However, grade I foci within the grade II and III tumors retained gelsolin expression, as did areas exhibiting squamous differentiation, regardless of the grade of the glandular component. Normal ovarian surface epithelium and all 4 ovarian cystadenomas expressed gelsolin, whereas 13 of 13 serous and mucinous carcinomas were negative. In contrast, 3 of 4 ovarian clear cell carcinomas were positive. The loss of gelsolin expression in ovarian and endometrial carcinomas is consistent with a tumor suppressor function for gelsolin, although its absence in proliferative glands and expression in squamous differentiation suggests a more complex relation than that previously reported for other tumors. Further investigation is needed to establish whether or not gelsolin expression provides diagnostic or prognostic information.

Original languageEnglish (US)
Pages (from-to)30-34
Number of pages5
JournalApplied Immunohistochemistry
Volume6
Issue number1
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Gelsolin
Microfilament Proteins
Adenocarcinoma
Neoplasms
Endometrial Hyperplasia
Endometrial Neoplasms
Actin Cytoskeleton
Cystadenoma
Mucinous Adenocarcinoma
Cytoskeleton
Hyperplasia
Ovary
Urinary Bladder
Epithelium

Keywords

  • Actin-binding proteins
  • Female genital tract neoplasms
  • Gelsolin
  • Tumor suppressors

ASJC Scopus subject areas

  • Anatomy
  • Medical Laboratory Technology

Cite this

Decreased expression of the actin-binding protein gelsolin in endometrial and ovarian adenocarcinomas. / Afify, Alaa M; Werness, Bruce A.

In: Applied Immunohistochemistry, Vol. 6, No. 1, 1998, p. 30-34.

Research output: Contribution to journalArticle

@article{eb4ee3fd117f46d9a883e6d47c051c4e,
title = "Decreased expression of the actin-binding protein gelsolin in endometrial and ovarian adenocarcinomas",
abstract = "Malignant cells are characterized by abnormalities of the cytoskeleton, and actin filaments in particular show marked disturbances of this normally well-organized network. Of the many actin-binding proteins, gelsolin plays a particularly important role in the remodeling of the actin cytoskeleton. Gelsolin expression is lost in transformed cells and in carcinomas of the breast and bladder, suggesting a role for gelsolin as a tumor suppressor. Because other human cancers have not yet been studied, we analyzed carcinomas of the endometrium and ovary for changes in gelsolin expression. Normal endometrial glands were positive in the secretory phase, whereas proliferative glands had lost expression. Although both simple and complex endometrial hyperplasias without atypia expressed gelsolin, some complex hyperplasias with atypia lost gelsolin expression. Expression in endometrial adenocarcinomas was related to grade; 11 of 14 grade II and III tumors were negative compared with 0 of 2 grade I tumors. However, grade I foci within the grade II and III tumors retained gelsolin expression, as did areas exhibiting squamous differentiation, regardless of the grade of the glandular component. Normal ovarian surface epithelium and all 4 ovarian cystadenomas expressed gelsolin, whereas 13 of 13 serous and mucinous carcinomas were negative. In contrast, 3 of 4 ovarian clear cell carcinomas were positive. The loss of gelsolin expression in ovarian and endometrial carcinomas is consistent with a tumor suppressor function for gelsolin, although its absence in proliferative glands and expression in squamous differentiation suggests a more complex relation than that previously reported for other tumors. Further investigation is needed to establish whether or not gelsolin expression provides diagnostic or prognostic information.",
keywords = "Actin-binding proteins, Female genital tract neoplasms, Gelsolin, Tumor suppressors",
author = "Afify, {Alaa M} and Werness, {Bruce A.}",
year = "1998",
doi = "10.1097/00022744-199803000-00006",
language = "English (US)",
volume = "6",
pages = "30--34",
journal = "Applied Immunohistochemistry and Molecular Morphology",
issn = "1062-3345",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Decreased expression of the actin-binding protein gelsolin in endometrial and ovarian adenocarcinomas

AU - Afify, Alaa M

AU - Werness, Bruce A.

PY - 1998

Y1 - 1998

N2 - Malignant cells are characterized by abnormalities of the cytoskeleton, and actin filaments in particular show marked disturbances of this normally well-organized network. Of the many actin-binding proteins, gelsolin plays a particularly important role in the remodeling of the actin cytoskeleton. Gelsolin expression is lost in transformed cells and in carcinomas of the breast and bladder, suggesting a role for gelsolin as a tumor suppressor. Because other human cancers have not yet been studied, we analyzed carcinomas of the endometrium and ovary for changes in gelsolin expression. Normal endometrial glands were positive in the secretory phase, whereas proliferative glands had lost expression. Although both simple and complex endometrial hyperplasias without atypia expressed gelsolin, some complex hyperplasias with atypia lost gelsolin expression. Expression in endometrial adenocarcinomas was related to grade; 11 of 14 grade II and III tumors were negative compared with 0 of 2 grade I tumors. However, grade I foci within the grade II and III tumors retained gelsolin expression, as did areas exhibiting squamous differentiation, regardless of the grade of the glandular component. Normal ovarian surface epithelium and all 4 ovarian cystadenomas expressed gelsolin, whereas 13 of 13 serous and mucinous carcinomas were negative. In contrast, 3 of 4 ovarian clear cell carcinomas were positive. The loss of gelsolin expression in ovarian and endometrial carcinomas is consistent with a tumor suppressor function for gelsolin, although its absence in proliferative glands and expression in squamous differentiation suggests a more complex relation than that previously reported for other tumors. Further investigation is needed to establish whether or not gelsolin expression provides diagnostic or prognostic information.

AB - Malignant cells are characterized by abnormalities of the cytoskeleton, and actin filaments in particular show marked disturbances of this normally well-organized network. Of the many actin-binding proteins, gelsolin plays a particularly important role in the remodeling of the actin cytoskeleton. Gelsolin expression is lost in transformed cells and in carcinomas of the breast and bladder, suggesting a role for gelsolin as a tumor suppressor. Because other human cancers have not yet been studied, we analyzed carcinomas of the endometrium and ovary for changes in gelsolin expression. Normal endometrial glands were positive in the secretory phase, whereas proliferative glands had lost expression. Although both simple and complex endometrial hyperplasias without atypia expressed gelsolin, some complex hyperplasias with atypia lost gelsolin expression. Expression in endometrial adenocarcinomas was related to grade; 11 of 14 grade II and III tumors were negative compared with 0 of 2 grade I tumors. However, grade I foci within the grade II and III tumors retained gelsolin expression, as did areas exhibiting squamous differentiation, regardless of the grade of the glandular component. Normal ovarian surface epithelium and all 4 ovarian cystadenomas expressed gelsolin, whereas 13 of 13 serous and mucinous carcinomas were negative. In contrast, 3 of 4 ovarian clear cell carcinomas were positive. The loss of gelsolin expression in ovarian and endometrial carcinomas is consistent with a tumor suppressor function for gelsolin, although its absence in proliferative glands and expression in squamous differentiation suggests a more complex relation than that previously reported for other tumors. Further investigation is needed to establish whether or not gelsolin expression provides diagnostic or prognostic information.

KW - Actin-binding proteins

KW - Female genital tract neoplasms

KW - Gelsolin

KW - Tumor suppressors

UR - http://www.scopus.com/inward/record.url?scp=0031895663&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031895663&partnerID=8YFLogxK

U2 - 10.1097/00022744-199803000-00006

DO - 10.1097/00022744-199803000-00006

M3 - Article

AN - SCOPUS:0031895663

VL - 6

SP - 30

EP - 34

JO - Applied Immunohistochemistry and Molecular Morphology

JF - Applied Immunohistochemistry and Molecular Morphology

SN - 1062-3345

IS - 1

ER -