Decreased expression of bcl-2 and bcl-x mRNA coincides with apoptosis following intracerebral administration of 3-nitropropionic acid

Shuzo Sato, Glenn T. Gobbel, Jari Honkaniemi, Yibing Li, Takeo Kondo, Kensuke Murakami, Minako Sato, Jean Christophe Copin, Frank R Sharp, Pak H. Chan

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The mitochondrial toxin, 3-nitropropionic acid (3-NP), is an irreversible inhibitor of succinate dehydrogenase that induces apoptosis in vitro and in vivo. We injected 3-NP into the striatum of rats to examine the potential role of Bcl-2 or Bcl-x, proteins that can inhibit apoptosis, in brain injury due to 3-NP. Electrophoretic examination of striatal tissue indicated that 3-NP induced internucleosomal fragmentation typical of apoptosis. There was also histologic evidence of apoptosis based on staining by the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNEL) method. Apoptosis was first observed 6 h after injection, was maximal at 1 day, and was still observed on day 7. Expression of bcl-2, bcl-x, and c-jun mRNA expression was evaluated 1, 3, 6, and 12 h and 1, 3, 5, and 7 days after injection using in situ hybridization. Both bcl-2 and bcl-x mRNA expression in the striatum decreased starting at 6 h and continued to 5 days after injection. This was in contrast to an apparent increase in c-jun expression. The similarity in the time course of apoptosis to that of suppression of bcl-2 and bcl-x mRNA suggests that changes in expression of these genes may contribute to apoptosis following 3-NP injection.

Original languageEnglish (US)
Pages (from-to)56-64
Number of pages9
JournalBrain Research
Volume808
Issue number1
DOIs
StatePublished - Oct 12 1998
Externally publishedYes

Fingerprint

Apoptosis
Messenger RNA
Injections
Corpus Striatum
Succinate Dehydrogenase
DNA Nucleotidylexotransferase
Biotin
3-nitropropionic acid
Brain Injuries
In Situ Hybridization
Staining and Labeling
Gene Expression
Proteins

Keywords

  • 3-Nitropropionic acid
  • Apoptosis
  • bcl- 2
  • bcl-x
  • c-jun
  • Huntington's disease
  • Mitochondria

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Decreased expression of bcl-2 and bcl-x mRNA coincides with apoptosis following intracerebral administration of 3-nitropropionic acid. / Sato, Shuzo; Gobbel, Glenn T.; Honkaniemi, Jari; Li, Yibing; Kondo, Takeo; Murakami, Kensuke; Sato, Minako; Copin, Jean Christophe; Sharp, Frank R; Chan, Pak H.

In: Brain Research, Vol. 808, No. 1, 12.10.1998, p. 56-64.

Research output: Contribution to journalArticle

Sato, S, Gobbel, GT, Honkaniemi, J, Li, Y, Kondo, T, Murakami, K, Sato, M, Copin, JC, Sharp, FR & Chan, PH 1998, 'Decreased expression of bcl-2 and bcl-x mRNA coincides with apoptosis following intracerebral administration of 3-nitropropionic acid', Brain Research, vol. 808, no. 1, pp. 56-64. https://doi.org/10.1016/S0006-8993(98)00784-7
Sato, Shuzo ; Gobbel, Glenn T. ; Honkaniemi, Jari ; Li, Yibing ; Kondo, Takeo ; Murakami, Kensuke ; Sato, Minako ; Copin, Jean Christophe ; Sharp, Frank R ; Chan, Pak H. / Decreased expression of bcl-2 and bcl-x mRNA coincides with apoptosis following intracerebral administration of 3-nitropropionic acid. In: Brain Research. 1998 ; Vol. 808, No. 1. pp. 56-64.
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