Decreased endothelial nitric oxide synthase expression and function contribute to impaired mitochondrial biogenesis and oxidative stress in fetal lambs with persistent pulmonary hypertension

Adeleye J. Afolayan, Annie Eis, Maxwell Alexander, Teresa Michalkiewicz, Ru Jeng Teng, Satyanarayana Lakshminrusimha, Girija G. Konduri

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Impaired vasodilation in persistent pulmonary hypertension of the newborn (PPHN) is characterized by mitochondrial dysfunction. We investigated the hypothesis that a decreased endothelial nitric oxide synthase level leads to impaired mitochondrial biogenesis and function in a lamb model of PPHN induced by prenatal ductus arteriosus constriction. We ventilated PPHN lambs with 100% O2 alone or with inhaled nitric oxide (iNO). We treated pulmonary artery endothelial cells (PAECs) from normal and PPHN lambs with detaNONOate, an NO donor. We observed decreased mitochondrial (mt) DNA copy number, electron transport chain (ETC) complex subunit levels, and ATP levels in PAECs and lung tissue of PPHN fetal lambs at baseline compared with gestation matched controls. Phosphorylation of AMP-activated kinase (AMPK) and levels of peroxisome proliferator-activated receptorcoactivator 1-(PGC-1) and sirtuin-1, which facilitate mitochondrial biogenesis, were decreased in PPHN. Ventilation with 100% O2 was associated with larger decreases in ETC subunits in the lungs of PPHN lambs compared with unventilated PPHN lambs. iNO administration, which facilitated weaning of FIO 2, partly restored mtDNA copy number, ETC subunit levels, and ATP levels. DetaNONOate increased eNOS phosphorylation and its interaction with heat shock protein 90 (HSP90); increased levels of superoxide dismutase 2 (SOD2) mRNA, protein, and activity; and decreased the mitochondrial superoxide levels in PPHN-PAECs. Knockdown of eNOS decreased ETC protein levels in control PAECs. We conclude that ventilation with 100% O2 amplifies oxidative stress and mitochondrial dysfunction in PPHN, which are partly improved by iNO and weaning of oxygen.

Original languageEnglish (US)
Pages (from-to)L40-L49
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume310
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

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Persistent Fetal Circulation Syndrome
Nitric Oxide Synthase Type III
Organelle Biogenesis
Pulmonary Hypertension
Oxidative Stress
Electron Transport
Pulmonary Artery
Endothelial Cells
Nitric Oxide
Weaning
Mitochondrial DNA
Ventilation
Sirtuin 1
Adenosine Triphosphate
Phosphorylation
Peroxisome Proliferators
HSP90 Heat-Shock Proteins
Ductus Arteriosus
Lung
AMP-Activated Protein Kinases

Keywords

  • Biogenesis
  • ENOS
  • Mitochondria
  • Oxidative stress
  • SOD2

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Decreased endothelial nitric oxide synthase expression and function contribute to impaired mitochondrial biogenesis and oxidative stress in fetal lambs with persistent pulmonary hypertension. / Afolayan, Adeleye J.; Eis, Annie; Alexander, Maxwell; Michalkiewicz, Teresa; Teng, Ru Jeng; Lakshminrusimha, Satyanarayana; Konduri, Girija G.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 310, No. 1, 01.01.2016, p. L40-L49.

Research output: Contribution to journalArticle

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AU - Afolayan, Adeleye J.

AU - Eis, Annie

AU - Alexander, Maxwell

AU - Michalkiewicz, Teresa

AU - Teng, Ru Jeng

AU - Lakshminrusimha, Satyanarayana

AU - Konduri, Girija G.

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