Deciphering the role of mucosal immune responses and the cervicovaginal microbiome in resistance to HIV infection in HIV-exposed seronegative (HESN) women

Sivasankaran Munusamy Ponnan, Kannan Thiruvengadam, Chaitanya Tellapragada, Anoop T. Ambikan, Aswathy Narayanan, Sujitha Kathirvel, Manikannan Mathayan, Janani Shankar, Akshaya Rajaraman, Mehar Afshan Amanulla, Thongadi Ramesh Dinesha, Selvamuthu Poongulali, Shanmugam Saravanan, Kailapuri Gangatharan Murugavel, Soumya Swaminathan, Vijayakumar Velu, Barbara Shacklett, Ujjwal Neogi, Luke Elizabeth Hanna

Research output: Contribution to journalArticlepeer-review

Abstract

The female genital tract (FGT) is an important site of human immunodeficiency virus (HIV) infection. Discerning the nature of HIV-specific local immune responses is crucial for identifying correlates of protection in HIV-exposed seronegative (HESN) individuals. The present study involved a comprehensive analysis of soluble immune mediators, secretory immunoglobulins (sIg), natural killer (NK) cells, CXCR51 CD81 T cells, T follicular helper (Tfh) cells, and T regulatory cells (Tregs) in the vaginal mucosa as well as the nature and composition of the cervicovaginal microbiome in HESN women. We found significantly elevated antiviral cytokines, soluble immunoglobulins, and increased frequencies of activated NK cells, CXCR51 CD81 T cells, and Tfh cells in HESN females compared to HIV-unexposed healthy (UH) women. Analysis of the genital microbiome of HESN women revealed a greater bacterial diversity and increased abundance of Gardnerella spp. in the mucosa. The findings suggest that the female genital tract of HESN females represents a microenvironment equipped with innate immune factors, antiviral mediators, and critical T cell subsets that protect against HIV infection. IMPORTANCE The vast majority of human immunodeficiency virus (HIV) infections across the world occur via the sexual route. The genital tract mucosa is thus the primary site of HIV replication, and discerning the nature of HIV-specific immune responses in this compartment is crucial. The role of the innate immune system at the mucosal level in exposed seronegative individuals and other HIV controllers remains largely unexplored. This understanding can provide valuable insights to improve vaccine design. We investigated mucosal T follicular helper (Tfh) cells, CXCR51 CD81 T cells, natural killer (NK) cells subsets, soluble immune markers, and microbiome diversity in HIV-exposed seronegative (HESN) women. We found a significantly higher level of mucosal CXCR51 CD81 T cells, CD41 Tfh cells, activated NK cell subsets, and antiviral immune cell mediators in HESN women. We also found a higher abundance of Gardnerella spp., microbiome dysbiosis, and decreased levels of inflammatory markers to be associated with reduced susceptibility to HIV infection. Our findings indicate that increased distribution of mucosal NK cells, CXCR51 CD81 T cells, Tfh cells, and soluble markers in HIV controllers with a highly diverse cervicovaginal microbiome could contribute effectively to protection against HIV infection. Overall, our findings imply that future vaccine design should emphasize inducing these highly functional cell types at the mucosal sites.

Original languageEnglish (US)
Article numbere00470-21
JournalMicrobiology spectrum
Volume9
Issue number2
DOIs
StatePublished - Oct 2021

Keywords

  • B cells
  • CBA
  • Cervicovaginal lavage
  • Cervicovaginal microbiota
  • CXCR5 CD8 cells
  • Cytobrush
  • HESN
  • Memory B cells
  • NK cells
  • Tfh cells
  • TSCM cells

ASJC Scopus subject areas

  • Physiology
  • Ecology
  • Immunology and Microbiology(all)
  • Genetics
  • Microbiology (medical)
  • Cell Biology
  • Infectious Diseases

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