Deciphering the Growth Behaviour of Mycobacterium africanum

Florian Gehre, Jacob Otu, Kathryn DeRiemer, Paola Florez de Sessions, Martin L. Hibberd, Wim Mulders, Tumani Corrah, Bouke C. de Jong, Martin Antonio

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background:Human tuberculosis (TB) in West Africa is not only caused by M. tuberculosis but also by bacteria of the two lineages of M. africanum. For instance, in The Gambia, 40% of TB is due to infections with M. africanum West African 2. This bacterial lineage is associated with HIV infection, reduced ESAT-6 immunogenicity and slower progression to active disease. Although these characteristics suggest an attenuated phenotype of M. africanum, no underlying mechanism has been described. From the first descriptions of M. africanum in the literature in 1969, the time to a positive culture of M. africanum on solid medium was known to be longer than the time to a positive culture of M. tuberculosis. However, the delayed growth of M. africanum, which may correlate with the less virulent phenotype in the human host, has not previously been studied in detail.Methodology/Principal Findings:We compared the growth rates of M. tuberculosis and M. africanum isolates from The Gambia in two liquid culture systems. M. africanum grows significantly slower than M. tuberculosis, not only when grown directly from sputa, but also in growth experiments under defined laboratory conditions. We also sequenced four M. africanum isolates and compared their whole genomes with the published M. tuberculosis H37Rv genome. M. africanum strains have several non-synonymous SNPs or frameshift mutations in genes that were previously associated with growth-attenuation. M. africanum strains also have a higher mutation frequency in genes crucial for transport of sulphur, ions and lipids/fatty acids across the cell membrane into the bacterial cell. Surprisingly, 5 of 7 operons, recently described as essential for intracellular survival of H37Rv in the host macrophage, showed at least one non-synonymously mutated gene in M. africanum.Conclusions/Significance:The altered growth behaviour of M. africanum might indicate a different survival strategy within host cells.

Original languageEnglish (US)
Article numbere2220
JournalPLoS Neglected Tropical Diseases
Volume7
Issue number5
DOIs
StatePublished - May 2013

Fingerprint

Mycobacterium
Tuberculosis
Growth
Gambia
Genome
Genes
Phenotype
Frameshift Mutation
Western Africa
Survival
Ion Transport
Mutation Rate
Operon
Sputum
Sulfur
HIV Infections
Single Nucleotide Polymorphism
Fatty Acids
Macrophages
Cell Membrane

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Gehre, F., Otu, J., DeRiemer, K., de Sessions, P. F., Hibberd, M. L., Mulders, W., ... Antonio, M. (2013). Deciphering the Growth Behaviour of Mycobacterium africanum. PLoS Neglected Tropical Diseases, 7(5), [e2220]. https://doi.org/10.1371/journal.pntd.0002220

Deciphering the Growth Behaviour of Mycobacterium africanum. / Gehre, Florian; Otu, Jacob; DeRiemer, Kathryn; de Sessions, Paola Florez; Hibberd, Martin L.; Mulders, Wim; Corrah, Tumani; de Jong, Bouke C.; Antonio, Martin.

In: PLoS Neglected Tropical Diseases, Vol. 7, No. 5, e2220, 05.2013.

Research output: Contribution to journalArticle

Gehre, F, Otu, J, DeRiemer, K, de Sessions, PF, Hibberd, ML, Mulders, W, Corrah, T, de Jong, BC & Antonio, M 2013, 'Deciphering the Growth Behaviour of Mycobacterium africanum', PLoS Neglected Tropical Diseases, vol. 7, no. 5, e2220. https://doi.org/10.1371/journal.pntd.0002220
Gehre F, Otu J, DeRiemer K, de Sessions PF, Hibberd ML, Mulders W et al. Deciphering the Growth Behaviour of Mycobacterium africanum. PLoS Neglected Tropical Diseases. 2013 May;7(5). e2220. https://doi.org/10.1371/journal.pntd.0002220
Gehre, Florian ; Otu, Jacob ; DeRiemer, Kathryn ; de Sessions, Paola Florez ; Hibberd, Martin L. ; Mulders, Wim ; Corrah, Tumani ; de Jong, Bouke C. ; Antonio, Martin. / Deciphering the Growth Behaviour of Mycobacterium africanum. In: PLoS Neglected Tropical Diseases. 2013 ; Vol. 7, No. 5.
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