Decellularized rhesus monkey kidney as a three-dimensional scaffold for renal tissue engineering

Karina H. Nakayama, Cynthia A. Batchelder, Charles C Lee, Alice F Tarantal

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

The goal of this study was the production of a decellularized kidney scaffold with structural, mechanical, and physiological properties necessary for engineering basic renal structures in vitro. Fetal, infant, juvenile, and adult rhesus monkey kidney sections were treated with either 1% (v/v) sodium dodecyl sulfate or Triton X-100 followed by quantitative and qualitative analysis. Comparison of decellularization agents and incubation temperatures demonstrated sodium dodecyl sulfate at 4°C to be most effective in preserving the native architecture. Hematoxylin and eosin staining confirmed the removal of cellular material, and immunohistochemistry demonstrated preservation of native expression patterns of extracellular matrix proteins, including heparan sulfate proteoglycan, fibronectin, collagen types I and IV, and laminin. Biomechanical testing revealed a decrease in the compressive modulus of decellularized compared to fresh kidneys. Layering of fetal kidney explants on age-matched decellularized kidney scaffolds demonstrated the capacity of the scaffold to support Pax2+/vimentin+ cell attachment and migration to recellularize the scaffold. These findings demonstrate that decellularized kidney sections retain critical structural and functional properties necessary for use as a three-dimensional scaffold and promote cellular repopulation. Further, this study provides the initial steps in developing new regenerative medicine strategies for renal tissue engineering and repair.

Original languageEnglish (US)
Pages (from-to)2207-2216
Number of pages10
JournalTissue Engineering - Part A
Volume16
Issue number7
DOIs
StatePublished - Jul 1 2010

Fingerprint

Tissue Engineering
Scaffolds (biology)
Macaca mulatta
Tissue engineering
Scaffolds
Kidney
Sodium dodecyl sulfate
Sodium Dodecyl Sulfate
Heparan Sulfate Proteoglycans
Collagen Type IV
Extracellular Matrix Proteins
Octoxynol
Laminin
Vimentin
Hematoxylin
Eosine Yellowish-(YS)
Collagen Type I
Fibronectins
Collagen
Repair

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Decellularized rhesus monkey kidney as a three-dimensional scaffold for renal tissue engineering. / Nakayama, Karina H.; Batchelder, Cynthia A.; Lee, Charles C; Tarantal, Alice F.

In: Tissue Engineering - Part A, Vol. 16, No. 7, 01.07.2010, p. 2207-2216.

Research output: Contribution to journalArticle

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