DC electric fields induce rapid directional migration in cultured human corneal epithelial cells

Behnom Farboud, Richard Nuccitelli, Ivan R. Schwab, Roslyn Rivkah Isseroff

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

After an epithelium is wounded, multiple soluble and extracellular matrix-associated signals induce a repair response. An often-overlooked signal is the endogenous electrical field established in the vicinity of the wound immediately upon disruption of epithelial integrity. Previous studies have detected lateral electric fields of approximately 42 mV mm-1 near bovine corneal wounds. In addition, electric fields on the order of 100-200 mV mm-1 have been measured lateral to wounds in mammalian epidermis. Here we report the migratory response of human corneal epithelial cells to DC electric fields of similar, physiologic magnitude. Our findings demonstrate that in a 100 mV mm-1 DC field, corneal epithelial cells demonstrate directed migration towards the cathode. The migratory speed and distances traversed by cultured human corneal epithelial cells is remarkably similar to those of cultured skin-derived keratinocytes under similar conditions: however, corneal epithelial cells demonstrate a more rapid directional response to the field than keratinocytes. These findings suggest that endogenous, wound-induced electric fields present in the cornea play an important role in human corneal wound healing by orienting the directional response of migratory cells so that they efficiently re-epithelialize the wounded area. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)667-673
Number of pages7
JournalExperimental Eye Research
Volume70
Issue number5
DOIs
StatePublished - 2000

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Epithelial Cells
Wounds and Injuries
Keratinocytes
Epidermis
Wound Healing
Cornea
Extracellular Matrix
Electrodes
Epithelium
Skin

Keywords

  • Cell migration
  • Corneal epithelial cells
  • Image analysis
  • Keratinocytes
  • Wound healing

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

DC electric fields induce rapid directional migration in cultured human corneal epithelial cells. / Farboud, Behnom; Nuccitelli, Richard; Schwab, Ivan R.; Isseroff, Roslyn Rivkah.

In: Experimental Eye Research, Vol. 70, No. 5, 2000, p. 667-673.

Research output: Contribution to journalArticle

Farboud, Behnom ; Nuccitelli, Richard ; Schwab, Ivan R. ; Isseroff, Roslyn Rivkah. / DC electric fields induce rapid directional migration in cultured human corneal epithelial cells. In: Experimental Eye Research. 2000 ; Vol. 70, No. 5. pp. 667-673.
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