Dackel acts in the ectoderm of the zebrafish pectoral fin bud to maintain AER signaling

H. Grandel, B. W. Draper, S. Schulte-Merker

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Classical embryological studies have implied the existence of an apical ectodermal maintenance factor (AEMF) that sustains signaling from the apical ectodermal ridge (AER) during vertebrate limb development. Recent evidence suggests that AEMF activity is composed of different signals involving both a sonic hedgehog (Shh) signal and a fibroblast growth factor 10 (Fgf10) signal from the mesenchyme. In this study we show that the product of the dackel (dak) gene is one of the components that acts in the epidermis of the zebrafish pectoral fin bud to maintain signaling from the apical fold, which is homologous to the AER of tetrapods. dak acts synergistically with Shh to induce fgf4 and fgf8 expression but independently of Shh in promoting apical fold morphogenesis. The failure of dak mutant fin buds to progress from the initial fin induction phase to the autonomous outgrowth phase causes loss of both AER and Shh activity, and subsequently results in a proximodistal truncation of the fin, similar to the result obtained by ridge ablation experiments in the chicken. Further analysis of the dak mutant phenotype indicates that the activity of the transcription factor engrailed 1 (En1) in the ventral non-ridge ectoderm also depends on a maintenance signal probably provided by the ridge. This results uncovers a new interaction between the AER and the dorsoventral organizer in the zebrafish pectoral fin bud.

Original languageEnglish (US)
Pages (from-to)4169-4178
Number of pages10
JournalDevelopment
Volume127
Issue number19
StatePublished - 2000
Externally publishedYes

Fingerprint

Ectoderm
Hedgehogs
Zebrafish
Maintenance
Fibroblast Growth Factor 10
Mesoderm
Morphogenesis
Epidermis
Vertebrates
Chickens
Transcription Factors
Extremities
Phenotype
Genes

Keywords

  • Dackel
  • Dorsoventral axis
  • Limb
  • Pectoral fin
  • Zebrafis

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Grandel, H., Draper, B. W., & Schulte-Merker, S. (2000). Dackel acts in the ectoderm of the zebrafish pectoral fin bud to maintain AER signaling. Development, 127(19), 4169-4178.

Dackel acts in the ectoderm of the zebrafish pectoral fin bud to maintain AER signaling. / Grandel, H.; Draper, B. W.; Schulte-Merker, S.

In: Development, Vol. 127, No. 19, 2000, p. 4169-4178.

Research output: Contribution to journalArticle

Grandel, H, Draper, BW & Schulte-Merker, S 2000, 'Dackel acts in the ectoderm of the zebrafish pectoral fin bud to maintain AER signaling', Development, vol. 127, no. 19, pp. 4169-4178.
Grandel H, Draper BW, Schulte-Merker S. Dackel acts in the ectoderm of the zebrafish pectoral fin bud to maintain AER signaling. Development. 2000;127(19):4169-4178.
Grandel, H. ; Draper, B. W. ; Schulte-Merker, S. / Dackel acts in the ectoderm of the zebrafish pectoral fin bud to maintain AER signaling. In: Development. 2000 ; Vol. 127, No. 19. pp. 4169-4178.
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