D-Lactate production as a function of glucose metabolism in Saccharomyces cerevisiae

Benjamin J. Stewart, Ali Navid, Kristen S. Kulp, Jennifer L S Knaack, Graham Bench

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Methylglyoxal, a reactive, toxic dicarbonyl, is generated by the spontaneous degradation of glycolytic intermediates. Methylglyoxal can form covalent adducts with cellular macromolecules, potentially disrupting cellular function. We performed experiments using the model organism Saccharomyces cerevisiae, grown in media containing low, moderate and high glucose concentrations, to determine the relationship between glucose consumption and methylglyoxal metabolism. Normal growth experiments and glutathione depletion experiments showed that metabolism of methylglyoxal by log-phase yeast cultured aerobically occurred primarily through the glyoxalase pathway. Growth in high-glucose media resulted in increased generation of the methylglyoxal metabolite d-lactate and overall lower efficiency of glucose utilization as measured by growth rates. Cells grown in high-glucose media maintained higher glucose uptake flux than cells grown in moderate-glucose or low-glucose media. Computational modelling showed that increased glucose consumption may impair catabolism of triose phosphates as a result of an altered NAD+:NADH ratio.

Original languageEnglish (US)
Pages (from-to)81-91
Number of pages11
JournalYeast
Volume30
Issue number2
DOIs
StatePublished - Feb 2013
Externally publishedYes

Keywords

  • d-lactate
  • Glycation
  • Glycolysis, NAD
  • Methylglyoxal

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Bioengineering
  • Applied Microbiology and Biotechnology

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    Stewart, B. J., Navid, A., Kulp, K. S., Knaack, J. L. S., & Bench, G. (2013). D-Lactate production as a function of glucose metabolism in Saccharomyces cerevisiae. Yeast, 30(2), 81-91. https://doi.org/10.1002/yea.2942