Abstract
Background & Aims: 5-Hydroxytryptamine (5-HT) is released from enterochromaffin cells and activates neural reflex programs regulating motility and secretion. Although sugars are reported to release 5-HT in vivo, it is unclear whether they act directly on enterochromaffin cells or indirectly through an intermediary messenger. The aim was to determine if D-glucose is a stimulus for 5-HT release. Methods: Human BON cells, derived from enterochromaffin cells, were treated with D-glucose, galactose, and the nonmetabolizable methyl α-D-glucopyranoside, or with fructose. Results: Reverse-transcription polymerase chain reaction together with Western blot analysis revealed an SGLT-like protein. D-Glucose caused a concentration-dependent increase in 5-HT release, which was mimicked by methyl α-D-glucopyranoside and galactose but not fructose. D-Glucose-stimulated 5-HT release was significantly reduced by phloridzin. Concentrations of mannitol below 75 mmol/L were ineffective in releasing 5-HT. Brefeldin A abolished forskolin-stimulated 5-HT release without affecting basal or constitutive release. Conclusions: The results show that high concentrations of metabolizable and nonmetabolizable hexoses activate signal transduction pathways, leading to release of 5-HT. These findings imply a role for enterochromaffin cells as "glucose sensors" during ingestion of a meal.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1400-1406 |
| Number of pages | 7 |
| Journal | Gastroenterology |
| Volume | 121 |
| Issue number | 6 |
| State | Published - 2001 |
Fingerprint
ASJC Scopus subject areas
- Gastroenterology
Cite this
D-glucose releases 5-hydroxytryptamine from human BON cells as a model of enterochromaffin cells. / Kim, Minsoo; Cooke, Helen J.; Javed, Najma H.; Carey, Hannah V.; Christofi, Fievos; Raybould, Helen E.
In: Gastroenterology, Vol. 121, No. 6, 2001, p. 1400-1406.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - D-glucose releases 5-hydroxytryptamine from human BON cells as a model of enterochromaffin cells
AU - Kim, Minsoo
AU - Cooke, Helen J.
AU - Javed, Najma H.
AU - Carey, Hannah V.
AU - Christofi, Fievos
AU - Raybould, Helen E
PY - 2001
Y1 - 2001
N2 - Background & Aims: 5-Hydroxytryptamine (5-HT) is released from enterochromaffin cells and activates neural reflex programs regulating motility and secretion. Although sugars are reported to release 5-HT in vivo, it is unclear whether they act directly on enterochromaffin cells or indirectly through an intermediary messenger. The aim was to determine if D-glucose is a stimulus for 5-HT release. Methods: Human BON cells, derived from enterochromaffin cells, were treated with D-glucose, galactose, and the nonmetabolizable methyl α-D-glucopyranoside, or with fructose. Results: Reverse-transcription polymerase chain reaction together with Western blot analysis revealed an SGLT-like protein. D-Glucose caused a concentration-dependent increase in 5-HT release, which was mimicked by methyl α-D-glucopyranoside and galactose but not fructose. D-Glucose-stimulated 5-HT release was significantly reduced by phloridzin. Concentrations of mannitol below 75 mmol/L were ineffective in releasing 5-HT. Brefeldin A abolished forskolin-stimulated 5-HT release without affecting basal or constitutive release. Conclusions: The results show that high concentrations of metabolizable and nonmetabolizable hexoses activate signal transduction pathways, leading to release of 5-HT. These findings imply a role for enterochromaffin cells as "glucose sensors" during ingestion of a meal.
AB - Background & Aims: 5-Hydroxytryptamine (5-HT) is released from enterochromaffin cells and activates neural reflex programs regulating motility and secretion. Although sugars are reported to release 5-HT in vivo, it is unclear whether they act directly on enterochromaffin cells or indirectly through an intermediary messenger. The aim was to determine if D-glucose is a stimulus for 5-HT release. Methods: Human BON cells, derived from enterochromaffin cells, were treated with D-glucose, galactose, and the nonmetabolizable methyl α-D-glucopyranoside, or with fructose. Results: Reverse-transcription polymerase chain reaction together with Western blot analysis revealed an SGLT-like protein. D-Glucose caused a concentration-dependent increase in 5-HT release, which was mimicked by methyl α-D-glucopyranoside and galactose but not fructose. D-Glucose-stimulated 5-HT release was significantly reduced by phloridzin. Concentrations of mannitol below 75 mmol/L were ineffective in releasing 5-HT. Brefeldin A abolished forskolin-stimulated 5-HT release without affecting basal or constitutive release. Conclusions: The results show that high concentrations of metabolizable and nonmetabolizable hexoses activate signal transduction pathways, leading to release of 5-HT. These findings imply a role for enterochromaffin cells as "glucose sensors" during ingestion of a meal.
UR - http://www.scopus.com/inward/record.url?scp=0035207186&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035207186&partnerID=8YFLogxK
M3 - Article
VL - 121
SP - 1400
EP - 1406
JO - Gastroenterology
JF - Gastroenterology
SN - 0016-5085
IS - 6
ER -