Cytoplasmic strains and strain rates in motile polymorphonuclear leukocytes

S. I. Simon, G. W. Schmid-Schonbein

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A new method is presented to measure local cytoplasmic deformation and rate of deformation in motile active neutrophils. The deformation is expressed in terms of biomechanical strains and strain rates. For this purpose small phagocytosed latex microspheres were used as intracellular markers. Planar Lagrangian and Eulerian strains and the rate of strain were estimated from the positions of a triad of internalized markers. Principal strains, stretch ratios, and principal directions were computed. The intracellular strains were found to be large relative to the overall cell shape change. Principal cytoplasmic stretch ratios showed large extension in the direction of pseudopod formation and cell locomotion and contraction in perpendicular directions. Regional strain analysis showed contractile strains to predominate in the vicinity of the pseudopod or leading edge of motion. The transitional region between the pseudopod and the main cell body exhibited large shear strains. Th posterior region, where the uropod is located, also revealed large extensions but small contratile strains. The rate of strains are relatively small, nonuniform in time, and largely independent of the strain. The method we propose to measure cytoplasmic strain can be applied to a variety of problems in cell mechanics.

Original languageEnglish (US)
Pages (from-to)319-332
Number of pages14
JournalBiophysical Journal
Volume58
Issue number2
StatePublished - 1990

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Pseudopodia
Neutrophils
Cell Shape
Latex
Mechanics
Microspheres
Phagocytosis
Cell Movement
Direction compound

ASJC Scopus subject areas

  • Biophysics

Cite this

Simon, S. I., & Schmid-Schonbein, G. W. (1990). Cytoplasmic strains and strain rates in motile polymorphonuclear leukocytes. Biophysical Journal, 58(2), 319-332.

Cytoplasmic strains and strain rates in motile polymorphonuclear leukocytes. / Simon, S. I.; Schmid-Schonbein, G. W.

In: Biophysical Journal, Vol. 58, No. 2, 1990, p. 319-332.

Research output: Contribution to journalArticle

Simon, SI & Schmid-Schonbein, GW 1990, 'Cytoplasmic strains and strain rates in motile polymorphonuclear leukocytes', Biophysical Journal, vol. 58, no. 2, pp. 319-332.
Simon, S. I. ; Schmid-Schonbein, G. W. / Cytoplasmic strains and strain rates in motile polymorphonuclear leukocytes. In: Biophysical Journal. 1990 ; Vol. 58, No. 2. pp. 319-332.
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